DNA bending potentials for loop-mediated nucleosome repositioning

• Verfasst von: Biswas, Mithun [VerfasserIn]
• Verfasst von: Langowski, Jörg [VerfasserIn]
• Verfasst von: Smith, Jeremy C. [VerfasserIn]
• Titel: DNA bending potentials for loop-mediated nucleosome repositioning
• Verf.angabe: M. Biswas, T. Wocjan, J. Langowski and J.C. Smith
• E-Jahr: 2012
• Jahr: 6 February 2012
• Umfang: 6 S.
• Fussnoten: Gesehen am 17.08.2018
• Titel Quelle: Enthalten in: epl
• Ort Quelle: Les Ulis : EDP Sciences, 1986
• Jahr Quelle: 2012
• Band/Heft Quelle: 97(2012,3) Artikel-Nummer 38004, 6 Seiten
• ISSN Quelle: 1286-4854
• DOI: doi:10.1209/0295-5075/97/38004
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1580137229

Abstract

Nucleosome repositioning is a fundamental process in gene function. DNA elasticity is a key element of loop-mediated nucleosome repositioning. Two analytical models for DNA elasticity have been proposed: the linear sub-elastic chain (SEC), which allows DNA kinking, and the worm-like chain (WLC), with a harmonic bending potential. In vitro studies have shown that nucleosomes reposition in a discontiguous manner on a segment of DNA and this has also been found in ground-state calculations with the WLC analytical model. Here we study using Monte Carlo simulation the dynamics of DNA loop-mediated nucleosome repositioning at physiological temperatures using the SEC and WLC potentials. At thermal energies both models predict nearest-neighbor repositioning of nucleosomes on DNA, in contrast to the repositioning in jumps observed in experiments. This suggests a crucial role of DNA sequence in nucleosome repositioning.