Rif2 promotes a telomere fold-back structure through Rpd3L recruitment in budding yeast

• Verfasst von: Poschke, Heiko [VerfasserIn]
• Verfasst von: Dees, Martina [VerfasserIn]
• Verfasst von: Kaderali, Lars [VerfasserIn]
• Verfasst von: Luke, Brian [VerfasserIn]
• Titel: Rif2 promotes a telomere fold-back structure through Rpd3L recruitment in budding yeast
• Verf.angabe: Heiko Poschke, Martina Dees, Michael Chang, Sandeep Amberkar, Lars Kaderali, Rodney Rothstein, Brian Luke
• Fussnoten: Gesehen am 24.08.2018
• Titel Quelle: Enthalten in: Public Library of Science: PLoS Genetics
• Jahr Quelle: 2012
• Band/Heft Quelle: 8(2012,9) Artikel-Nummer e1002960, 13 Seiten
• ISSN Quelle: 1553-7404
• DOI: doi:10.1371/journal.pgen.1002960
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1580375294

Abstract

Using a genome-wide screening approach, we have established the genetic requirements for proper telomere structure in Saccharomyces cerevisiae. We uncovered 112 genes, many of which have not previously been implicated in telomere function, that are required to form a fold-back structure at chromosome ends. Among other biological processes, lysine deacetylation, through the Rpd3L, Rpd3S, and Hda1 complexes, emerged as being a critical regulator of telomere structure. The telomeric-bound protein, Rif2, was also found to promote a telomere fold-back through the recruitment of Rpd3L to telomeres. In the absence of Rpd3 function, telomeres have an increased susceptibility to nucleolytic degradation, telomere loss, and the initiation of premature senescence, suggesting that an Rpd3-mediated structure may have protective functions. Together these data reveal that multiple genetic pathways may directly or indirectly impinge on telomere structure, thus broadening the potential targetsavailable to manipulate telomere function.