Amphiregulin antibody and reduction of axial elongation in experimental myopia

• Verfasst von: Jiang, Wen Jun [VerfasserIn]
• Verfasst von: Jonas, Jost B. [VerfasserIn]
• Titel: Amphiregulin antibody and reduction of axial elongation in experimental myopia
• Verf.angabe: Wen Jun Jiang, Hui Xin Song, Shao Yu Li, Bin Guo, Jian Feng Wu, Guo Ping Li, Da Dong Guo, De Long Shi, Hong Sheng Bi, Jost B. Jonas
• E-Jahr: 2017
• Jahr: 23 February 2017
• Umfang: 11 S.
• Fussnoten: Available online 23 February 2017 ; Gesehen am 27.08.2018
• Titel Quelle: Enthalten in: EBioMedicine
• Ort Quelle: Amsterdam [u.a.] : Elsevier, 2014
• Jahr Quelle: 2017
• Band/Heft Quelle: 17(2017), Seite 134-144
• ISSN Quelle: 2352-3964
• DOI: doi:10.1016/j.ebiom.2017.02.021
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Amphiregulin
• Sach-SW: Axial elongation
• Sach-SW: Epithelial growth factor
• Sach-SW: Experimental myopia
• Sach-SW: Myopia
• K10plus-PPN: 1580406378

Abstract

To examine the mechanism of ocular axial elongation in myopia, guinea pigs (age: 2-3weeks) which either underwent unilateral or bilateral lens-induced myopization (group 1) or which were primarily myopic at baseline (group 2) received unilateral intraocular injections of amphiregulin antibody (doses: 5, 10, or 15μg) three times in intervals of 9days. A third group of emmetropic guinea pigs got intraocular unilateral injections of amphiregulin (doses: 0.25, 0.50 or 1.00ng, respectively). In each group, the contralateral eyes received intraocular injections of Ringer's solution. In intra-animal inter-eye comparison and intra-eye follow-up comparison in groups 1 and 2, the study eyes as compared to the contralateral eyes showed a dose-dependent reduction in axial elongation. In group 3, study eyes and control eyes did not differ significantly in axial elongation. Immunohistochemistry revealed amphiregulin labelling at the retinal pigment epithelium in eyes with lens-induced myopization and Ringer's solution injection, but not in eyes with amphiregulin antibody injection. Intraocular injections of amphiregulin-antibody led to a reduction of lens-induced axial myopic elongation and of the physiological eye enlargement in young guinea pigs. In contrast, intraocularly injected amphiregulin in a dose of ≤1ng did not show a significant effect. Amphiregulin may be one of several essential molecular factors for axial elongation.