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Status: Bibliographieeintrag

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Verfasst von:Augustin, Iris [VerfasserIn]   i
 Kerr, Grainne [VerfasserIn]   i
 Boutros, Michael [VerfasserIn]   i
Titel:Autocrine Wnt regulates the survival and genomic stability of embryonic stem cells
Verf.angabe:Iris Augustin, Dyah L. Dewi, Jennifer Hundshammer, Gerrit Erdmann, Grainne Kerr, and Michael Boutros
E-Jahr:2017
Jahr:10 January 2017
Umfang:?
Fussnoten:Gesehen am 28.08.2018
Titel Quelle:Enthalten in: Science signaling
Ort Quelle:Washington, DC [u.a.] : Assoc., 2008
Jahr Quelle:2017
Band/Heft Quelle:10(2017,461) Artikel-Nummer eaah6829
ISSN Quelle:1937-9145
Abstract:Wnt signaling plays an important role in the self-renewal and differentiation of stem cells. The secretion of Wnt ligands requires Evi (also known as Wls). Genetically ablating Evi provides an experimental approach to studying the consequence of depleting all redundant Wnt proteins, and overexpressing Evi enables a nonspecific means of increasing Wnt signaling. We generated Evi-deficient and Evi-overexpressing mouse embryonic stem cells (ESCs) to analyze the role of autocrine Wnt production in self-renewal and differentiation. Self-renewal was reduced in Evi-deficient ESCs and increased in Evi-overexpressing ESCs in the absence of leukemia inhibitory factor, which supports the self-renewal of ESCs. The differentiation of ESCs into cardiomyocytes was enhanced when Evi was overexpressed and teratoma formation and growth of Evi-deficient ESCs in vivo were impaired, indicating that autocrine Wnt ligands were necessary for ESC differentiation and survival. ESCs lacking autocrine Wnt signaling had mitotic defects and showed genomic instability. Together, our study demonstrates that autocrine Wnt secretion is important for the survival, chromosomal stability, differentiation, and tumorigenic potential of ESCs.
DOI:doi:10.1126/scisignal.aah6829
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: http://dx.doi.org/10.1126/scisignal.aah6829
 DOI: https://doi.org/10.1126/scisignal.aah6829
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Animals
 Autocrine Communication
 Cell Differentiation
 Cell Proliferation
 Cell Self Renewal
 Cell Survival
 Cells, Cultured
 Gene Expression Profiling
 Genomic Instability
 HEK293 Cells
 Humans
 Intracellular Signaling Peptides and Proteins
 Mice
 Mice, 129 Strain
 Mice, Inbred C57BL
 Mice, Knockout
 Mouse Embryonic Stem Cells
 Receptors, G-Protein-Coupled
 Wnt Proteins
 Wnt Signaling Pathway
K10plus-PPN:1580447856
Verknüpfungen:→ Zeitschrift

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