| Online-Ressource |
Verfasst von: | Munteanu, Bogdan [VerfasserIn]  |
| Reitzenstein, Carolina von [VerfasserIn]  |
| Hänsch, Gertrud Maria [VerfasserIn]  |
| Meyer, Björn [VerfasserIn]  |
| Hopf, Carsten [VerfasserIn]  |
Titel: | Sensitive, robust and automated protein analysis of cell differentiation and of primary human blood cells by intact cell MALDI mass spectrometry biotyping |
Verf.angabe: | Bogdan Munteanu, Carolina von Reitzenstein, Gertrud Maria Hänsch, Björn Meyer, Carsten Hopf |
E-Jahr: | 2012 |
Jahr: | 06 September 2012 |
Umfang: | 10 S. |
Fussnoten: | Gesehen am 29.08.2018 |
Titel Quelle: | Enthalten in: Analytical and bioanalytical chemistry |
Ort Quelle: | Berlin : Springer, 2002 |
Jahr Quelle: | 2012 |
Band/Heft Quelle: | 404(2012), 8, Seite 2277-2286 |
ISSN Quelle: | 1618-2650 |
Abstract: | Intact cell mass spectrometry biotyping, a collection of methods for classification of cells based on mass spectrometric fingerprints, is an established method in clinical and environmental microbiology. It has recently also been applied to the investigation of mammalian cells including primary blood cells and cultured cells. However, few automated procedures suitable for higher throughput and little analytical standardization of mammalian biotyping approaches have been reported so far. Here, we present a novel automated method that robustly classifies as few as 250 cells per spot. Automatically acquired cell fingerprints from cultured and primary cells show high technical (R > 0.95) and biological reproducibility (R = 0.83-0.96), with a median peak variance below 12 %. Ion suppression is shown to be a major concern at higher cell numbers and needs to be carefully monitored. We demonstrate that intact cell mass spectrometric signatures of different cell lines start to resemble each other at higher trifluoroacetic acid (TFA) concentrations and that therefore low concentrations of TFA in the matrix solution are preferred. We show that in vitro differentiation of HL-60 cells into a neutrophil-like phenotype can be rapidly and robustly monitored. We utilize the method for global analysis of person-to-person differences in mass spectral signatures of intact polymorphonuclear neutrophils and monocytes obtained from healthy volunteers. Our data suggest that automated MALDI mass spectrometry cell biotyping could be a useful complementary approach in clinical cell analysis. Open image in new window Figure Sensitive, robust and automated MALDI mass spectrometry biotyping enables analysis of cell differentiation fingerprints |
DOI: | doi:10.1007/s00216-012-6357-0 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: http://dx.doi.org/10.1007/s00216-012-6357-0 |
| Volltext: https://doi.org/10.1007/s00216-012-6357-0 |
| DOI: https://doi.org/10.1007/s00216-012-6357-0 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Bibliogr. Hinweis: | Erscheint auch als : Druck-Ausgabe: Munteanu, Bogdan, 1985 - : Sensitive, robust and automated protein analysis of cell differentiation and of primary human blood cells by intact cell MALDI mass spectrometry biotyping. - 2012 |
Sach-SW: | Cell differentiation |
| Intact cell mass spectrometry |
| MALDI |
| Mammalian cell biotyping |
| Primary blood cells |
K10plus-PPN: | 158048297X |
Verknüpfungen: | → Zeitschrift |
Sensitive, robust and automated protein analysis of cell differentiation and of primary human blood cells by intact cell MALDI mass spectrometry biotyping / Munteanu, Bogdan [VerfasserIn]; 06 September 2012 (Online-Ressource)