Navigation überspringen
Universitätsbibliothek Heidelberg
Status: Bibliographieeintrag

Verfügbarkeit
Standort: ---
Exemplare: ---
heiBIB
 Online-Ressource
Verfasst von:Munteanu, Bogdan [VerfasserIn]   i
 Reitzenstein, Carolina von [VerfasserIn]   i
 Hänsch, Gertrud Maria [VerfasserIn]   i
 Meyer, Björn [VerfasserIn]   i
 Hopf, Carsten [VerfasserIn]   i
Titel:Sensitive, robust and automated protein analysis of cell differentiation and of primary human blood cells by intact cell MALDI mass spectrometry biotyping
Verf.angabe:Bogdan Munteanu, Carolina von Reitzenstein, Gertrud Maria Hänsch, Björn Meyer, Carsten Hopf
E-Jahr:2012
Jahr:06 September 2012
Umfang:10 S.
Fussnoten:Gesehen am 29.08.2018
Titel Quelle:Enthalten in: Analytical and bioanalytical chemistry
Ort Quelle:Berlin : Springer, 2002
Jahr Quelle:2012
Band/Heft Quelle:404(2012), 8, Seite 2277-2286
ISSN Quelle:1618-2650
Abstract:Intact cell mass spectrometry biotyping, a collection of methods for classification of cells based on mass spectrometric fingerprints, is an established method in clinical and environmental microbiology. It has recently also been applied to the investigation of mammalian cells including primary blood cells and cultured cells. However, few automated procedures suitable for higher throughput and little analytical standardization of mammalian biotyping approaches have been reported so far. Here, we present a novel automated method that robustly classifies as few as 250 cells per spot. Automatically acquired cell fingerprints from cultured and primary cells show high technical (R > 0.95) and biological reproducibility (R = 0.83-0.96), with a median peak variance below 12 %. Ion suppression is shown to be a major concern at higher cell numbers and needs to be carefully monitored. We demonstrate that intact cell mass spectrometric signatures of different cell lines start to resemble each other at higher trifluoroacetic acid (TFA) concentrations and that therefore low concentrations of TFA in the matrix solution are preferred. We show that in vitro differentiation of HL-60 cells into a neutrophil-like phenotype can be rapidly and robustly monitored. We utilize the method for global analysis of person-to-person differences in mass spectral signatures of intact polymorphonuclear neutrophils and monocytes obtained from healthy volunteers. Our data suggest that automated MALDI mass spectrometry cell biotyping could be a useful complementary approach in clinical cell analysis. Open image in new window Figure Sensitive, robust and automated MALDI mass spectrometry biotyping enables analysis of cell differentiation fingerprints
DOI:doi:10.1007/s00216-012-6357-0
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: http://dx.doi.org/10.1007/s00216-012-6357-0
 Volltext: https://doi.org/10.1007/s00216-012-6357-0
 DOI: https://doi.org/10.1007/s00216-012-6357-0
Datenträger:Online-Ressource
Sprache:eng
Bibliogr. Hinweis:Erscheint auch als : Druck-Ausgabe: Munteanu, Bogdan, 1985 - : Sensitive, robust and automated protein analysis of cell differentiation and of primary human blood cells by intact cell MALDI mass spectrometry biotyping. - 2012
Sach-SW:Cell differentiation
 Intact cell mass spectrometry
 MALDI
 Mammalian cell biotyping
 Primary blood cells
K10plus-PPN:158048297X
Verknüpfungen:→ Zeitschrift

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/68299936   QR-Code
zum Seitenanfang