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Verfasst von:Eisner, Christoph [VerfasserIn]   i
Titel:Measurement of plasma volume using fluorescent silica-based nanoparticles
Verf.angabe:Christoph Eisner, Hooisweng Ow, Tianxin Yang, Zhanjun Jia, Emilios Dimitriadis, Lingli Li, Kenneth Wang, Josephine Briggs, Mark Levine, Jurgen Schnermann, Michael Graham Espey
Jahr:2012
Jahr des Originals:2011
Umfang:7 S.
Fussnoten:First published December 15, 2011 ; Gesehen am 29.08.2018
Titel Quelle:Enthalten in: Journal of applied physiology
Ort Quelle:Bethesda, Md. : American Physiological Society, 1948
Jahr Quelle:2012
Band/Heft Quelle:112(2012), 4, Seite 681-687
ISSN Quelle:1522-1601
Abstract:Plasma volume (PV) is an important determinant of cardiovascular function and organ perfusion, and it is the target of infusion and diuretic therapies in daily clinical practice. Despite its fundamental importance PV is not commonly measured because available methods of tracer dilution are reliant on dye substances that suffer from numerous drawbacks including binding plasma proteins, spectral changes, and clearance kinetics that complicate analysis and interpretation. To address these issues, we have tested the utility of fluorescent nanoparticles comprised of a dye-rich silica core and polyethylene glycol-coated shell. Photophysical and visual analysis showed discrete size-gradated nanoparticle populations could be synthesized within a distribution tolerance of ±4 nm, which were optically unaffected in the presence of plasma/albumin. In normal mice, the cutoff for renal filtration of nanoparticles from blood into urine was ≤11 nm. A linear relationship between body weight and PV was readily determined in mice administered far red fluorescent nanoparticles sized either 20 or 30 nm. PV measurements using nanoparticles were correlated to values obtained with Evans blue dye. Induced expansion or contraction of PV was demonstrated with albumin or furosemide administration, respectively, in mice. Longitudinal experiments >30 min required matched untreated control mice to correct for nanoparticle loss (≈30%) putatively to the reticuloendothelial/phagocyte system. Collectively, the findings support a nanotechnology-based solution to methodological problems in measure of PV, notably in clinical settings where information on hemodynamic changes may improve treatment of injury and disease.
DOI:doi:10.1152/japplphysiol.01068.2011
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

kostenfrei: Volltext: https://www.physiology.org/doi/10.1152/japplphysiol.01068.2011
 kostenfrei: Volltext: http://dx.doi.org/10.1152/japplphysiol.01068.2011
 DOI: https://doi.org/10.1152/japplphysiol.01068.2011
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1580523382
Verknüpfungen:→ Zeitschrift

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