| Online-Ressource |
Verfasst von: | Dräger, Nina [VerfasserIn]  |
| Nachman, Eliana [VerfasserIn]  |
| Shah, Pranav [VerfasserIn]  |
| Katsinelos, Taxiarchis [VerfasserIn]  |
| Boulant, Steeve [VerfasserIn]  |
| Teleman, Aurelio A. [VerfasserIn]  |
| Jahn, Thomas R. [VerfasserIn]  |
Titel: | Bin1 directly remodels actin dynamics through its BAR domain |
Verf.angabe: | Nina M. Dräger, Eliana Nachman, Moritz Winterhoff, Stefan Brühmann, Pranav Shah, Taxiarchis Katsinelos, Steeve Boulant, Aurelio A. Teleman, Jan Faix, Thomas R. Jahn |
E-Jahr: | 2017 |
Jahr: | 11.09.2017 |
Umfang: | 16 S. |
Fussnoten: | Gesehen am 30.08.2018 |
Titel Quelle: | Enthalten in: European Molecular Biology OrganizationEMBO reports |
Ort Quelle: | Heidelberg : EMBO Press, 2000 |
Jahr Quelle: | 2017 |
Band/Heft Quelle: | 18(2017), 11, Seite 2051-2066 |
ISSN Quelle: | 1469-3178 |
Abstract: | Endocytic processes are facilitated by both curvature‐generating BAR‐domain proteins and the coordinated polymerization of actin filaments. Under physiological conditions, the N‐BAR protein Bin1 has been shown to sense and curve membranes in a variety of cellular processes. Recent studies have identified Bin1 as a risk factor for Alzheimer's disease, although its possible pathological function in neurodegeneration is currently unknown. Here, we report that Bin1 not only shapes membranes, but is also directly involved in actin binding through its BAR domain. We observed a moderate actin bundling activity by human Bin1 and describe its ability to stabilize actin filaments against depolymerization. Moreover, Bin1 is also involved in stabilizing tau‐induced actin bundles, which are neuropathological hallmarks of Alzheimer's disease. We also provide evidence for this effect in vivo, where we observed that downregulation of Bin1 in a Drosophila model of tauopathy significantly reduces the appearance of tau‐induced actin inclusions. Together, these findings reveal the ability of Bin1 to modify actin dynamics and provide a possible mechanistic connection between Bin1 and tau‐induced pathobiological changes of the actin cytoskeleton. Synopsis <img class="highwire-embed" alt="Embedded Image" src="http://embor.embopress.org/sites/default/files/highwire/embor/18/11/2051/embed/graphic-1.gif"/>. The membrane‐bending protein Bin1 has been identified as a risk factor for Alzheimer's disease. This study shows that Bin1 directly binds and stabilizes actin through its BAR domain, providing a possible link to tau‐induced pathological changes of the actin cytoskeleton. Different Bin1 isoforms and its Drosophila orthologues bind actin filaments through their BAR domains.Bin1 stabilizes actin filaments against depolymerization and moderately bundles actin filaments.Bin1 also stabilizes tau‐induced actin bundles in vitro and its downregulation reduces actin rods in a Drosophila tauopathy model. |
DOI: | doi:10.15252/embr.201744137 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: http://dx.doi.org/10.15252/embr.201744137 |
| Volltext: http://embor.embopress.org/content/18/11/2051 |
| DOI: https://doi.org/10.15252/embr.201744137 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | actin binding |
| Alzheimer's disease |
| genetic risk factor |
| N‐BAR protein Bin1 |
| tau |
K10plus-PPN: | 1580542204 |
Verknüpfungen: | → Zeitschrift |
Bin1 directly remodels actin dynamics through its BAR domain / Dräger, Nina [VerfasserIn]; 11.09.2017 (Online-Ressource)