| |  Online-Ressource |
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| Verfasst von: | Pilla, Esther [VerfasserIn]  |
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| | Melchior, Frauke [VerfasserIn] |
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| Titel: | A novel SUMO1-specific interacting motif in dipeptidyl peptidase 9 (DPP9) that Is important for enzymatic regulation |
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| Verf.angabe: | Esther Pilla, Ulrike Möller, Guido Sauer, Francesca Mattiroli, Frauke Melchior, and Ruth Geiss-Friedlander |
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| E-Jahr: | 2012 |
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| Jahr: | November 14, 2012 |
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| Umfang: | 10 S. |
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| Fussnoten: | Gesehen am 30.08.2018 |
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| Titel Quelle: | Enthalten in: The journal of biological chemistry |
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| Ort Quelle: | Bethesda, Md. : Soc., 1905 |
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| Jahr Quelle: | 2012 |
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| Band/Heft Quelle: | 287(2012), 53, Seite 44320-44329 |
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| ISSN Quelle: | 1083-351X |
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| Abstract: | Sumoylation affects many cellular processes by regulating the interactions of modified targets with downstream effectors. Here we identified the cytosolic dipeptidyl peptidase 9 (DPP9) as a SUMO1 interacting protein. Surprisingly, DPP9 binds to SUMO1 independent of the well known SUMO interacting motif, but instead interacts with a loop involving Glu67 of SUMO1. Intriguingly, DPP9 selectively associates with SUMO1 and not SUMO2, due to a more positive charge in the SUMO1-loop. We mapped the SUMO-binding site of DPP9 to an extended arm structure, predicted to directly flank the substrate entry site. Importantly, whereas mutants in the SUMO1-binding arm are less active compared with wild-type DPP9, SUMO1 stimulates DPP9 activity. Consistent with this, silencing of SUMO1 leads to a reduced cytosolic prolyl-peptidase activity. Taken together, these results suggest that SUMO1, or more likely, a sumoylated protein, acts as an allosteric regulator of DPP9. |
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| DOI: | doi:10.1074/jbc.M112.397224 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Kostenfrei: Volltext ; Verlag: http://dx.doi.org/10.1074/jbc.M112.397224 |
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| | Kostenfrei: Volltext: http://www.jbc.org/content/287/53/44320 |
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| | DOI: https://doi.org/10.1074/jbc.M112.397224 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Dipeptidyl Peptidase |
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| | Peptidases |
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| | Post-translational Modification |
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| | Protease |
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| | SUMO |
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| | SUMO-interacting Motif (SIM) |
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| | Sumoylation |
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| | Ubiquitin |
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| K10plus-PPN: | 158055654X |
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| Verknüpfungen: | → Zeitschrift |
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¬A¬ novel SUMO1-specific interacting motif in dipeptidyl peptidase 9 (DPP9) that Is important for enzymatic regulation / Pilla, Esther [VerfasserIn]; November 14, 2012 (Online-Ressource)
