Evolution of an intricate J-protein network driving protein disaggregation in eukaryotes

• Verfasst von: Nillegoda, Nadinath B. [VerfasserIn]
• Verfasst von: Stank, Antonia [VerfasserIn]
• Verfasst von: Alberts, Niels [VerfasserIn]
• Verfasst von: Szlachcic, Anna [VerfasserIn]
• Verfasst von: Wade, Rebecca C. [VerfasserIn]
• Verfasst von: Bukau, Bernd [VerfasserIn]
• Titel: Evolution of an intricate J-protein network driving protein disaggregation in eukaryotes
• Verf.angabe: Nadinath B Nillegoda, Antonia Stank, Duccio Malinverni, Niels Alberts, Anna Szlachcic, Alessandro Barducci, Paolo De Los Rios, Rebecca C Wade, Bernd Bukau
• E-Jahr: 2017
• Jahr: 15 May 2017
• Fussnoten: Gesehen am 31.08.2018
• Titel Quelle: Enthalten in: eLife
• Ort Quelle: Cambridge : eLife Sciences Publications, 2012
• Jahr Quelle: 2017
• Band/Heft Quelle: 6(2017) Artikel-Nummer e24560, 28 Seiten
• ISSN Quelle: 2050-084X
• DOI: doi:10.7554/eLife.24560
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1580584446

Abstract

Hsp70 participates in a broad spectrum of protein folding processes extending from nascent chain folding to protein disaggregation. This versatility in function is achieved through a diverse family of J-protein cochaperones that select substrates for Hsp70. Substrate selection is further tuned by transient complexation between different classes of J-proteins, which expands the range of protein aggregates targeted by metazoan Hsp70 for disaggregation. We assessed the prevalence and evolutionary conservation of J-protein complexation and cooperation in disaggregation. We find the emergence of a eukaryote-specific signature for interclass complexation of canonical J-proteins. Consistently, complexes exist in yeast and human cells, but not in bacteria, and correlate with cooperative action in disaggregation in vitro. Signature alterations exclude some J-proteins from networking, which ensures correct J-protein pairing, functional network integrity and J-protein specialization. This fundamental change in J-protein biology during the prokaryote-to-eukaryote transition allows for increased fine-tuning and broadening of Hsp70 function in eukaryotes., DOI: http://dx.doi.org/10.7554/eLife.24560.001, All cells must maintain their proteins in a correctly folded shape to survive. The task of sustaining a healthy set of proteins has increased with the rise of complex life from prokaryotes (such as bacteria) that form simple single-celled organisms to eukaryotes (such as yeast, plants and multicellular animals). As a result of organisms ageing or acquiring genetic mutations, or under stressful conditions such as high temperature, proteins can lose their normal shape and clump together to form “aggregates”. These aggregates are potentially toxic to cells and have been linked to many human diseases including neurodegeneration and cancer., Cells contain molecular machines that help break down aggregates and subsequently recycle or rescue trapped proteins. Some of these machines are based around a protein called Hsp70, which can perform a wide range of protein folding processes. So-called J-proteins help Hsp70 to select aggregates to be targeted for break down. It used to be thought that different classes of J-proteins interacted with Hsp70 separately. However, in 2015, researchers showed that in humans, two different classes of J-proteins can bind to each other to form a “complex”, which has distinct aggregate selection properties., Now, Nillegoda et al. - including several of the researchers involved in the 2015 study - have examined the evolutionary history of these J-protein complexes. This revealed that different classes (A and B) of J-proteins first cooperated after prokaryotes and eukaryotes diverged from each other. In particular, the molecular machinery that breaks down aggregates in yeast cells - but not the machinery found in bacteria - depends on complexes formed from the two classes of J-proteins., Further investigation revealed that in humans, J-proteins have structural features that ensure they pair up correctly to perform unique activities. Furthermore, Nillegoda et al. suggest that cooperation between J-proteins may have enabled organisms such as humans - which contain over 40 distinct J-proteins - to carry out further specialized protein-folding tasks that do not occur in prokaryotes., Overall, the findings presented by Nillegoda et al. reveal another important layer to protein quality control in eukaryotic cells. The next step is to understand the possible roles of different J-protein complexes play in J-protein associated cellular protein quality control processes such as preventing protein aggregation, refolding or recycling abnormal proteins. This knowledge could ultimately be used to develop treatments for diseases and disorders in which protein aggregates form., DOI: http://dx.doi.org/10.7554/eLife.24560.002