| |  Online-Ressource |
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| Verfasst von: | Majewsky, Marius [VerfasserIn]  |
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| | Johann, Pascal-David [VerfasserIn] |
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| | Jones, David T. W. [VerfasserIn] |
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| | Pfister, Stefan [VerfasserIn] |
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| | Haefeli, Walter E. [VerfasserIn] |
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| | Burhenne, Jürgen [VerfasserIn] |
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| Titel: | Systematic identification of suspected anthelmintic benzimidazole metabolites using LC-MS/MS |
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| Verf.angabe: | Marius Majewsky, David Castel, Ludivine Le Dret, Pascal Johann, David T. Jones, Stefan M. Pfister, Walter E. Haefeli, Jürgen Burhenne |
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| Jahr: | 2018 |
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| Jahr des Originals: | 2017 |
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| Umfang: | 8 S. |
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| Fussnoten: | available online 28 December 2017 ; Gesehen am 03.09.2018 |
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| Titel Quelle: | Enthalten in: Journal of pharmaceutical and biomedical analysis |
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| Ort Quelle: | New York, NY [u.a.] : Science Direct, 1983 |
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| Jahr Quelle: | 2018 |
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| Band/Heft Quelle: | 151(2018), Seite 151-158 |
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| ISSN Quelle: | 1873-264X |
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| Abstract: | Metabolite reference standards are often not available, which results in a lack of MS/MS spectra for library matching. Consequently, the identification of suspected metabolites proves to be challenging. The present study aims at structurally elucidating the MS/MS fragmentation behavior of selected benzimidazole anthelmintics to theoretically predict characteristic product ions for rapid and systematic tentative metabolite identification. A set of common characteristic product ions was identified from accurate mass MS/MS experiments for five parent compounds. It was hypothesized that the mass shift of any metabolic transformation at the parent molecule also is observable in the mass spectrum of the corresponding metabolite. This was tested and verified with six metabolite reference standards and subsequently, formulated as a general prediction scheme. The approach was integrated into a rapid MSe QTOF workflow and tested in mouse plasma for mebendazole and its metabolites. The presented scheme allows the prediction of characteristic product ions for suspected unknown metabolites. These can be matched with measured product ions of suspected metabolites for tentative identification. The theoretically predicted spectra can contribute to the tentative identification of unknown compounds in non-target and suspect screening approaches. |
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| DOI: | doi:10.1016/j.jpba.2017.12.056 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: http://dx.doi.org/10.1016/j.jpba.2017.12.056 |
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| | Volltext: http://www.sciencedirect.com/science/article/pii/S0731708517328224 |
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| | DOI: https://doi.org/10.1016/j.jpba.2017.12.056 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Benzimidazoles |
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| | LC-MS/MS |
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| | Mass shift |
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| | Metabolites |
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| | Structure elucidation |
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| K10plus-PPN: | 1580611273 |
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| Verknüpfungen: | → Zeitschrift |
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Systematic identification of suspected anthelmintic benzimidazole metabolites using LC-MS/MS / Majewsky, Marius [VerfasserIn]; 2018 (Online-Ressource)
