LST8 regulates cell growth via target-of-rapamycin complex 2 (TORC2)

• Verfasst von: Wang, Tao [VerfasserIn]
• Verfasst von: Edgar, Bruce [VerfasserIn]
• Titel: LST8 regulates cell growth via target-of-rapamycin complex 2 (TORC2)
• Verf.angabe: Tao Wang, Rachel Blumhagen, Uyen Lao, Ying Kuo, and Bruce A. Edgar
• E-Jahr: 2012
• Jahr: 9 April 2012
• Umfang: 11 S.
• Fussnoten: Gesehen am 04.09.2018
• Titel Quelle: Enthalten in: Molecular and cellular biology
• Ort Quelle: Washington, DC : Soc., 1981
• Jahr Quelle: 2012
• Band/Heft Quelle: 32(2012), 12, Seite 2203-2213
• ISSN Quelle: 1098-5549
• DOI: doi:10.1128/MCB.06474-11
• Datenträger: Online-Ressource
• Sprache: eng
• Bibliogr. Hinweis: Errata: Wang, Tao: Author's correction: LST8 regulates cell growth via target-of-rapamycin complex 2 (TORC2)
• K10plus-PPN: 1580669689

Abstract

The evolutionarily conserved serine/threonine protein kinase target-of-rapamycin (TOR) controls cell growth as a core component of TOR complexes 1 (TORC1) and 2 (TORC2). Although TORC1 is the more central growth regulator, TORC2 has also been shown to affect cell growth. Here, we demonstrate that Drosophila LST8, the only conserved TOR-binding protein present in both TORC1 and TORC2, functions exclusively in TORC2 and is not required for TORC1 activity. In mutants lacking LST8, expression of TOR and RAPTOR, together with their upstream activator Rheb, was sufficient to provide TORC1 activity and stimulate cell and organ growth. Furthermore, using an lst8 knockout mutation, we show that TORC2 regulates cell growth cell autonomously. Surprisingly, however, TORC2 does not regulate cell growth via its best-characterized target, AKT. Our findings support the possible application of TORC2-specific drugs in cancer therapy.