| |  Online-Ressource |
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| Verfasst von: | Knipping, Friederike [VerfasserIn]  |
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| | Petri, Karl [VerfasserIn] |
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| | Glimm, Hanno [VerfasserIn] |
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| | Kalle, Christof von [VerfasserIn] |
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| | Schmidt, Manfred [VerfasserIn] |
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| | Gabriel, Richard [VerfasserIn] |
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| Titel: | Genome-wide specificity of highly efficient TALENs and CRISPR/Cas9 for T cell receptor modification |
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| Verf.angabe: | Friederike Knipping, Mark J. Osborn, Karl Petri, Jakub Tolar, Hanno Glimm, Christof von Kalle, Manfred Schmidt, and Richard Gabriel |
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| E-Jahr: | 2017 |
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| Jahr: | 12 February 2017 |
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| Umfang: | 12 S. |
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| Fussnoten: | Available online 12 February 2017 ; Gesehen am 04.09.2018 |
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| Titel Quelle: | Enthalten in: Molecular therapy. Methods & clinical development |
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| Ort Quelle: | New York, NY : Nature Publishing Group, 2014 |
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| Jahr Quelle: | 2017 |
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| Band/Heft Quelle: | 4(2017), Seite 213-224 |
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| ISSN Quelle: | 2329-0501 |
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| Abstract: | In T cells with transgenic high-avidity T cell receptors (TCRs), endogenous and transferred TCR chains compete for surface expression and may pair inappropriately, potentially causing autoimmunity. To knock out endogenous TCR expression, we assembled 12 transcription activator-like effector nucleases (TALENs) and five guide RNAs (gRNAs) from the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas9) system. Using TALEN mRNA, TCR knockout was successful in up to 81% of T cells. Additionally, we were able to verify targeted gene addition of a GFP gene by homology-directed repair at the TALEN target site, using a donor suitable for replacement of the reporter transgene with therapeutic TCR chains. Remarkably, analysis of TALEN and CRISPR/Cas9 specificity using integrase-defective lentiviral vector capture revealed only one off-target site for one of the gRNAs and three off-target sites for both of the TALENs, indicating a high level of specificity. Collectively, our work shows highly efficient and specific nucleases for T cell engineering. |
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| DOI: | doi:10.1016/j.omtm.2017.01.005 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: http://dx.doi.org/10.1016/j.omtm.2017.01.005 |
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| | kostenfrei: Volltext: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5363317/ |
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| | DOI: https://doi.org/10.1016/j.omtm.2017.01.005 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1580672590 |
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| Verknüpfungen: | → Zeitschrift |
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Genome-wide specificity of highly efficient TALENs and CRISPR/Cas9 for T cell receptor modification / Knipping, Friederike [VerfasserIn]; 12 February 2017 (Online-Ressource)
