Navigation überspringen
Universitätsbibliothek Heidelberg
Status: Bibliographieeintrag

Verfügbarkeit
Standort: ---
Exemplare: ---
heiBIB
 Online-Ressource
Verfasst von:Weber, Laura [VerfasserIn]   i
 Jänisch, Thomas [VerfasserIn]   i
 Dübel, Stefan [VerfasserIn]   i
 Nesterov-Müller, Alexander [VerfasserIn]   i
 Breitling, Frank [VerfasserIn]   i
 Löffler, Felix [VerfasserIn]   i
Titel:Single amino acid fingerprinting of the human antibody repertoire with high density peptide arrays
Verf.angabe:Laura K. Weber, Andrea Palermo, Jonas Kügler, Olivier Armant, Awale Isse, Simone Rentschler, Thomas Jaenisch, Jürgen Hubbuch, Stefan Dübel, Alexander Nesterov-Mueller, Frank Breitling, Felix F. Loeffler
E-Jahr:2017
Jahr:3 February 2017
Umfang:10 S.
Fussnoten:Gesehen am 04.09.2018
Titel Quelle:Enthalten in: Journal of immunological methods
Ort Quelle:Amsterdam [u.a.] : Elsevier Science, 1971
Jahr Quelle:2017
Band/Heft Quelle:443(2017), Seite 45-54
ISSN Quelle:1872-7905
Abstract:The antibody species that patrol in a patient's blood are an invaluable part of the immune system. While most of them shield us from life-threatening infections, some of them do harm in autoimmune diseases. If we knew exactly all the antigens that elicited all the antibody species within a group of patients, we could learn which ones correlate with immune protection, are irrelevant, or do harm. Here, we demonstrate an approach to this question: First, we use a plethora of phage-displayed peptides to identify many different serum antibody binding peptides. Next, we synthesize identified peptides in the array format and rescreen the serum used for phage panning to validate antibody binding peptides. Finally, we systematically vary the sequence of validated antibody binding peptides to identify those amino acids within the peptides that are crucial for binding “their” antibody species. The resulting immune fingerprints can then be used to trace them back to potential antigens. We investigated the serum of an individual in this pipeline, which led to the identification of 73 antibody fingerprints. Some fingerprints could be traced back to their most likely antigen, for example the immunodominant capsid protein VP1 of enteroviruses, most likely elicited by the ubiquitous poliovirus vaccination. Thus, with our approach, it is possible, to pinpoint those antibody species that correlate with a certain antigen, without any pre-information. This can help to unravel hitherto enigmatic diseases.
DOI:doi:10.1016/j.jim.2017.01.012
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: http://dx.doi.org/10.1016/j.jim.2017.01.012
 Volltext: http://www.sciencedirect.com/science/article/pii/S0022175916302903
 DOI: https://doi.org/10.1016/j.jim.2017.01.012
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Humoral immune system
 Next generation sequencing
 Phage display
 Serology
 Substitution analysis
K10plus-PPN:1580676693
Verknüpfungen:→ Zeitschrift

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/68301981   QR-Code
zum Seitenanfang