Is it possible to detect PEth 16

• Verfasst von: Boll, Robert [VerfasserIn]
• Verfasst von: Johnson, Theron S. [VerfasserIn]
• Verfasst von: Kaaks, Rudolf [VerfasserIn]
• Verfasst von: Kühn, Tilman [VerfasserIn]
• Verfasst von: Skopp, Gisela [VerfasserIn]
• Titel: Is it possible to detect PEth 16
• Titelzusatz: 0/18:1 and PEth 18:1/18:1 in red blood cells after 20 years of storage in liquid nitrogen?
• Verf.angabe: Robert Boll, Theron Johnson, Rudolf Kaaks, Tilman Kühn, Gisela Skopp
• E-Jahr: 2017
• Jahr: 24 June 2017
• Umfang: 7 S.
• Fussnoten: Gesehen am 07.09.2018
• Titel Quelle: Enthalten in: International journal of legal medicine
• Ort Quelle: Berlin : Springer, 1922
• Jahr Quelle: 2017
• Band/Heft Quelle: 131(2017), 5, Seite 1291-1297
• ISSN Quelle: 1437-1596
• DOI: doi:10.1007/s00414-017-1626-2
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: EPIC-Heidelberg study
• Sach-SW: Habitual alcohol consumption
• Sach-SW: Long-term storage
• Sach-SW: Phosphatidylethanol (PEth)
• K10plus-PPN: 1580760325

Abstract

IntroductionPhosphatidylethanol (PEth), as measured in freshly drawn blood samples, could be a promising new biomarker for habitual alcohol consumption, but it is still unknown whether PEth can also be determined from blood samples having been stored frozen for a longer period.Materials and methodsPEth 16:0/18:1 and PEth 18:1/18:1 were determined by LC-MS/MS from red blood cells (RBC) derived from blood samples of (I) 20 healthy volunteers (after 1 month of storage at −80 °C) and (II) 232 participants of the population-based European Prospective Investigation into Cancer and Nutrition (EPIC)-Heidelberg study (after 20 years of storage at −196 °C). Analyses involved liquid-liquid extraction from 100 μl aliquots with phosphatidylpropanol (PProp 18:1/18:1) as the internal standard. Extracts were subjected to a 10-min LC gradient separation, using multiple reaction monitoring of deprotonated molecules for quantification.ResultsAfter 1 month of storage at −80 °C, PEth was detectable in all samples at mean concentrations of 393.6 ± 12.4 ng/ml (PEth 16:0/18:1) and 43.3 ± 1.1 ng/ml (PEth 18:1/18:1). In samples stored for 20 years at −196 °C, PEth was detectable in 23.7% of all samples at mean concentrations of 412.2 ± 655.5 ng/ml (PEth 16:0/18:1) and 38.0 ± 74.8 ng/ml (PEth 18:1/18:1).Discussion and conclusionPEth can be determined reliably from samples of moderate habitual alcohol consumers after 1 month of storage at −80 °C. Our data suggest that PEth is generally also detectable in samples after 20 years of storage at −196 °C. Further studies are needed to assess the still unknown impact of storage duration and temperature on different PEth specimen concentrations.