Status: Bibliographieeintrag
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| Online-Ressource |
Verfasst von: | Schirò, Giorgio [VerfasserIn]  |
| Schlichting, Ilme [VerfasserIn]  |
Titel: | Simple and efficient system for photoconverting light-sensitive proteins in serial crystallography experiments |
Verf.angabe: | Giorgio Schirò, Joyce Woodhouse, Martin Weik, Ilme Schlichting and Robert L. Shoeman |
Umfang: | 8 S. |
Fussnoten: | Gesehen am 12.09.2018 |
Titel Quelle: | Enthalten in: Journal of applied crystallography |
Jahr Quelle: | 2017 |
Band/Heft Quelle: | 50(2017), 3, S. 932-939 |
ISSN Quelle: | 1600-5767 |
Abstract: | Proteins that change their structure in response to light absorption regulate many functional processes in living cells. Moreover, biotechnological approaches like optogenetics and super-resolution fluorescence microscopy recently triggered the generation of new genetically modified photosensitive proteins. Light-induced structural changes in photosensitive proteins can be studied by time-resolved serial femtosecond crystallography (SFX), an X-ray diffraction technique that allows the determination of macromolecular structures at X-ray free-electron lasers from a large number of nano- to micro-sized crystals. This article describes a simple and efficient system for converting photosensitive proteins into light-induced semi-stationary states by inline laser illumination prior to sample injection with a gas-focused liquid jet and subsequent optical pump-X-ray probe exposure. The simple setup of this device makes it suitable for integration into other liquid injectors (like electro-spinning and electro-kinetic injectors) and potentially also in high-viscosity extruders, provided that embedding microcrystals in viscous media does not alter protein photophysical properties. The functioning of the device is demonstrated with an example of a photoswitchable fluorescent protein pre-illuminated (photoactivated) for time-resolved SFX experiments. The device can be easily adapted for the conversion in time-resolved SFX experiments of other microcrystalline proteins, such as photosystems, phytochromes and rhodopsins. |
DOI: | doi:10.1107/S1600576717006264 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Verlag: http://dx.doi.org/10.1107/S1600576717006264 |
| Verlag: https://onlinelibrary.wiley.com/doi/abs/10.1107/S1600576717006264 |
| DOI: https://doi.org/10.1107/S1600576717006264 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1580893716 |
Verknüpfungen: | → Zeitschrift |
Simple and efficient system for photoconverting light-sensitive proteins in serial crystallography experiments / Schirò, Giorgio [VerfasserIn] (Online-Ressource)
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