| |  Online-Ressource |
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| Verfasst von: | Simpson, Jeremy [VerfasserIn]  |
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| | Joggerst, Brigitte [VerfasserIn] |
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| | Laketa, Vibor [VerfasserIn] |
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| | Erfle, Holger [VerfasserIn] |
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| | Bechtel, Stephanie [VerfasserIn] |
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| | Wiemann, Stefan [VerfasserIn] |
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| | Ellenberg, Jan [VerfasserIn] |
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| | Pepperkok, Rainer [VerfasserIn] |
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| Titel: | Genome-wide RNAi screening identifies human proteins with a regulatory function in the early secretory pathway |
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| Verf.angabe: | Jeremy C. Simpson, Brigitte Joggerst, Vibor Laketa, Fatima Verissimo, Cihan Cetin, Holger Erfle, Mariana G. Bexiga, Vasanth R. Singan, Jean-Karim Hériché, Beate Neumann, Alvaro Mateos, Jonathon Blake, Stephanie Bechtel, Vladimir Benes, Stefan Wiemann, Jan Ellenberg and Rainer Pepperkok |
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| E-Jahr: | 2012 |
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| Jahr: | 3 June 2012 |
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| Umfang: | 11 S. |
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| Fussnoten: | Gesehen am 18.09.2018 |
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| Titel Quelle: | Enthalten in: Nature cell biology |
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| Ort Quelle: | New York, NY : Nature America, 1999 |
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| Jahr Quelle: | 2012 |
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| Band/Heft Quelle: | 14(2012), 7, Seite 764-774 |
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| ISSN Quelle: | 1476-4679 |
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| Abstract: | The secretory pathway in mammalian cells has evolved to facilitate the transfer of cargo molecules to internal and cell surface membranes. Use of automated microscopy-based genome-wide RNA interference screens in cultured human cells allowed us to identify 554 proteins influencing secretion. Cloning, fluorescent-tagging and subcellular localization analysis of 179 of these proteins revealed that more than two-thirds localize to either the cytoplasm or membranes of the secretory and endocytic pathways. The depletion of 143 of them resulted in perturbations in the organization of the COPII and/or COPI vesicular coat complexes of the early secretory pathway, or the morphology of the Golgi complex. Network analyses revealed a so far unappreciated link between early secretory pathway function, small GTP-binding protein regulation, actin cytoskeleton organization and EGF-receptor-mediated signalling. This work provides an important resource for an integrative understanding of global cellular organization and regulation of the secretory pathway in mammalian cells. |
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| DOI: | doi:10.1038/ncb2510 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: http://dx.doi.org/10.1038/ncb2510 |
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| | Volltext: https://www.nature.com/articles/ncb2510 |
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| | DOI: https://doi.org/10.1038/ncb2510 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1581068328 |
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| Verknüpfungen: | → Zeitschrift |
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Genome-wide RNAi screening identifies human proteins with a regulatory function in the early secretory pathway / Simpson, Jeremy [VerfasserIn]; 3 June 2012 (Online-Ressource)
