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Status: Bibliographieeintrag

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Verfasst von:Steck, Eric [VerfasserIn]   i
 Boeuf, Stephane [VerfasserIn]   i
 Gabler, Jessica [VerfasserIn]   i
 Werth, Nadine [VerfasserIn]   i
 Forró, Philipp Arne Florian [VerfasserIn]   i
 Diederichs, Solvig [VerfasserIn]   i
 Richter, Wiltrud [VerfasserIn]   i
Titel:Regulation of H19 and its encoded microRNA-675 in osteoarthritis and under anabolic and catabolic in vitro conditions
Verf.angabe:Eric Steck, Stephane Boeuf, Jessica Gabler, Nadine Werth, Philipp Schnatzer, Solvig Diederichs, Wiltrud Richter
E-Jahr:2012
Jahr:21 April 2012
Umfang:11 S.
Teil:volume:90
 year:2012
 number:10
 pages:1185-1195
 extent:11
Fussnoten:Gesehen am 20.09.2018
Titel Quelle:Enthalten in: Journal of molecular medicine
Ort Quelle:Berlin : Springer, 1922
Jahr Quelle:2012
Band/Heft Quelle:90(2012), 10, Seite 1185-1195
ISSN Quelle:1432-1440
Abstract:Cartilage degeneration in the course of osteoarthritis (OA) is associated with an alteration in chondrocyte metabolism. In order to identify molecules representing putative key regulators for diagnosis and therapeutic intervention, we analyzed gene expression and microRNA (miR) levels in OA and normal knee cartilage using a customized cartilage cDNA array and quantitative RT-PCR. Among newly identified candidate molecules, H19, IGF2, and ITM2A were significantly elevated in OA compared to normal cartilage. H19 is an imprinted maternally expressed gene influencing IGF2 expression, whose transcript is a long noncoding (lnc) RNA of unknown biological function harboring the miR-675. H19 and IGF2 mRNA levels did not correlate significantly within cartilage samples suggesting that deregulation by imprinting effects are unlikely. A significant correlation was, however, observed for H19, COL2A1, and miR-675 expression levels in OA tissue, and functional regulation of these candidate molecules was assessed under anabolic and catabolic conditions. Culture of chondrocytes under hypoxic signaling showed co-upregulation of H19, COL2A1, and miRNA-675 levels in close correlation. Proinflammatory cytokines IL-1β and TNF-α downregulated COL2A1, H19, and miR-675 significantly without close statistical correlation. In conclusion, this is the first report demonstrating deregulation of an lncRNA and its encoded miR in the context of OA-affected cartilage. Stress-induced regulation of H19 expression by hypoxic signaling and inflammation suggests that lncRNA H19 acts as a metabolic correlate in cartilage and cultured chondrocytes, while the miR-675 may indirectly influence COL2A1 levels. H19 may not only be an attractive marker for cell anabolism but also a potential target to stimulate cartilage recovery.
DOI:doi:10.1007/s00109-012-0895-y
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: http://dx.doi.org/10.1007/s00109-012-0895-y
 Volltext: https://doi.org/10.1007/s00109-012-0895-y
 DOI: https://doi.org/10.1007/s00109-012-0895-y
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Chondrocytes
 Gene expression
 Hypoxia
 Inflammation
 Long noncoding RNA (lncRNAs
 MicroRNA (miR)
K10plus-PPN:1581135866
Verknüpfungen:→ Zeitschrift

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