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Verfasst von:Steinkamp, Martin [VerfasserIn]   i
 Kirsch, Joachim [VerfasserIn]   i
Titel:Brain derived neurotrophic factor inhibits apoptosis in enteric glia during gut inflammation
Verf.angabe:Martin Steinkamp, Nadine Schulte, Ulrike Spaniol, Carolin Pflüger, Joachim Kirsch, Georg B. von Boyen
Umfang:6 S.
Fussnoten:Gesehen am 21.09.2018
Titel Quelle:Enthalten in: Medical science monitor
Jahr Quelle:2012
Band/Heft Quelle:18(2012), 4, S. BR117-BR122
ISSN Quelle:1643-3750
Abstract:Background: Enteric glia cells (EGCs) are essential for the integrity of the bowel. A loss of EGCs leads to a severe inflammation of the intestines. As a diminished EGC network is postulated in Crohn’s disease (CD), we aimed to investigate if EGCs could be a target of apoptosis during inflammation in CD, which can be influenced by Brain derived neurotrophic factor (BDNF). Material/Methods: GFAP, BDNF and cCaspase-3 were detected in the gut of patients with CD. Primary EGC cultures were established and cultivated. Tyrosine receptor kinase (TrkB) receptors on these cells were investigated by western blot and immunofluorescence. Rate of apoptosis was induced by tumor necrosis factor (TNF-alpha) and interferon (IFN-gamma). Apoptosis was determined by a fluorometric caspase 3/7 activation assay after preincubation of these cells with BDNF or neutralizing anti-BDNF antibodies. Results: Mucosal GFAP-positive EGCs undergo apoptosis revealed by cCaspase-3 in the gut of patients withCD expressing BDNF highly. The combination of TNF-alpha and IFN-gamma was able to induce apoptosis in primary EGCs, whereas these factors alone did not. Brain derived neurotrophic factor (BDNF) attenuate glia cell apoptosis to a small extent, but neutralizing antibodies against BDNF dramatically increased apoptosis. Conclusions: Mucosal EGC apoptosis is an important finding in the gut of patients with CD. Proinflammatory cytokines, which are highly increased in CD, induce EGC apoptosis, whereas the neurotrophin BDNF might be protective for EGC. Since EGCs are implicated in the maintenance of the enteric mucosal integrity, EGC apoptosis may contribute to the pathophysiological changes in CD.
DOI:doi:10.12659/MSM.882612
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Kostenfrei: Verlag: http://dx.doi.org/10.12659/MSM.882612
 Kostenfrei: Verlag: https://www.medscimonit.com/abstract/index/idArt/882612
 DOI: https://doi.org/10.12659/MSM.882612
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1581193769
Verknüpfungen:→ Zeitschrift

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