ATPase-dependent role of the atypical kinase Rio2 on the evolving pre-40S ribosomal subunit

• Verfasst von: Ferreira-Cerca, Sébastien [VerfasserIn]
• Verfasst von: Schäfer, Thorsten [VerfasserIn]
• Verfasst von: Wesseling, Anne-Maria [VerfasserIn]
• Verfasst von: Hurt, Ed [VerfasserIn]
• Titel: ATPase-dependent role of the atypical kinase Rio2 on the evolving pre-40S ribosomal subunit
• Verf.angabe: Sébastien Ferreira-Cerca, Vatsala Sagar, Thorsten Schäfer, Momar Diop, Anne-Maria Wesseling, Haiyun Lu, Eileen Chai, Ed Hurt & Nicole LaRonde-LeBlanc
• E-Jahr: 2012
• Jahr: 28 October 2012
• Umfang: 8 S.
• Fussnoten: Gesehen am 21.09.2018
• Titel Quelle: Enthalten in: Nature structural & molecular biology
• Ort Quelle: London [u.a.] : Nature Publishing Group, 2004
• Jahr Quelle: 2012
• Band/Heft Quelle: 19(2012), 12, Seite 1316-1323
• ISSN Quelle: 1545-9985
• DOI: doi:10.1038/nsmb.2403
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1581205066

Abstract

Ribosome synthesis involves dynamic association of ribosome-biogenesis factors with evolving preribosomal particles. Rio2 is an atypical protein kinase required for pre-40S subunit maturation. We report the crystal structure of eukaryotic Rio2-ATP-Mg2+ complex. The active site contains ADP-Mg2+ and a phosphoaspartate intermediate typically found in Na+, K+ and Ca2+ ATPases but not protein kinases. Consistent with this finding, ctRio2 exhibits a robust ATPase activity in vitro. In vivo, Rio2 docks on the ribosome, with its active site occluded and its flexible loop positioned to interact with the pre-40S subunit. Moreover, Rio2 catalytic activity is required for its dissociation from the ribosome, a necessary step in pre-40S maturation. We propose that phosphoryl transfer from ATP to Asp257 in Rio2's active site and subsequent hydrolysis of the aspartylphosphate could be a trigger to power late cytoplasmic 40S subunit biogenesis.