Status: Bibliographieeintrag
Standort: ---
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| Online-Ressource |
Verfasst von: | Ferreira-Cerca, Sébastien [VerfasserIn]  |
| Schäfer, Thorsten [VerfasserIn]  |
| Wesseling, Anne-Maria [VerfasserIn]  |
| Hurt, Ed [VerfasserIn]  |
Titel: | ATPase-dependent role of the atypical kinase Rio2 on the evolving pre-40S ribosomal subunit |
Verf.angabe: | Sébastien Ferreira-Cerca, Vatsala Sagar, Thorsten Schäfer, Momar Diop, Anne-Maria Wesseling, Haiyun Lu, Eileen Chai, Ed Hurt & Nicole LaRonde-LeBlanc |
E-Jahr: | 2012 |
Jahr: | 28 October 2012 |
Umfang: | 8 S. |
Fussnoten: | Gesehen am 21.09.2018 |
Titel Quelle: | Enthalten in: Nature structural & molecular biology |
Ort Quelle: | London [u.a.] : Nature Publishing Group, 2004 |
Jahr Quelle: | 2012 |
Band/Heft Quelle: | 19(2012), 12, Seite 1316-1323 |
ISSN Quelle: | 1545-9985 |
Abstract: | Ribosome synthesis involves dynamic association of ribosome-biogenesis factors with evolving preribosomal particles. Rio2 is an atypical protein kinase required for pre-40S subunit maturation. We report the crystal structure of eukaryotic Rio2-ATP-Mg2+ complex. The active site contains ADP-Mg2+ and a phosphoaspartate intermediate typically found in Na+, K+ and Ca2+ ATPases but not protein kinases. Consistent with this finding, ctRio2 exhibits a robust ATPase activity in vitro. In vivo, Rio2 docks on the ribosome, with its active site occluded and its flexible loop positioned to interact with the pre-40S subunit. Moreover, Rio2 catalytic activity is required for its dissociation from the ribosome, a necessary step in pre-40S maturation. We propose that phosphoryl transfer from ATP to Asp257 in Rio2's active site and subsequent hydrolysis of the aspartylphosphate could be a trigger to power late cytoplasmic 40S subunit biogenesis. |
DOI: | doi:10.1038/nsmb.2403 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: http://dx.doi.org/10.1038/nsmb.2403 |
| Volltext: https://www.nature.com/articles/nsmb.2403 |
| DOI: https://doi.org/10.1038/nsmb.2403 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1581205066 |
Verknüpfungen: | → Zeitschrift |
ATPase-dependent role of the atypical kinase Rio2 on the evolving pre-40S ribosomal subunit / Ferreira-Cerca, Sébastien [VerfasserIn]; 28 October 2012 (Online-Ressource)
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