The role of vimentin in regulating cell Invasive migration in dense cultures of breast carcinoma cells

• Verfasst von: Messica, Yonatan [VerfasserIn]
• Verfasst von: Lysakovskaia, Kseniia [VerfasserIn]
• Verfasst von: Eils, Roland [VerfasserIn]
• Verfasst von: Gladilin, Evgeny [VerfasserIn]
• Titel: The role of vimentin in regulating cell Invasive migration in dense cultures of breast carcinoma cells
• Verf.angabe: Yonatan Messica, Adi Laser-Azogui, Tova Volberg, Yair Elisha, Kseniia Lysakovskaia, Roland Eils, Evgeny Gladilin, Benjamin Geiger, Roy Beck
• E-Jahr: 2017
• Jahr: October 12, 2017
• Umfang: 8 S.
• Teil: volume:17
• Teil: year:2017
• Teil: number:11
• Teil: pages:6941-6948
• Teil: extent:8
• Fussnoten: Gesehen am 27.09.2018
• Titel Quelle: Enthalten in: Nano letters
• Ort Quelle: Washington, DC : ACS Publ., 2001
• Jahr Quelle: 2017
• Band/Heft Quelle: 17(2017), 11, Seite 6941-6948
• ISSN Quelle: 1530-6992
• DOI: doi:10.1021/acs.nanolett.7b03358
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 158136072X

Abstract

Cell migration and mechanics are tightly regulated by the integrated activities of the various cytoskeletal networks. In cancer cells, cytoskeletal modulations have been implicated in the loss of tissue integrity and acquisition of an invasive phenotype. In epithelial cancers, for example, increased expression of the cytoskeletal filament protein vimentin correlates with metastatic potential. Nonetheless, the exact mechanism whereby vimentin affects cell motility remains poorly understood. In this study, we measured the effects of vimentin expression on the mechano-elastic and migratory properties of the highly invasive breast carcinoma cell line MDA231. We demonstrate here that vimentin stiffens cells and enhances cell migration in dense cultures, but exerts little or no effect on the migration of sparsely plated cells. These results suggest that cell-cell interactions play a key role in regulating cell migration, and coordinating cell movement in dense cultures. Our findings pave the way toward understanding the relationship between cell migration and mechanics in a biologically relevant context.