Genome-wide analysis of the circulating miRNome after cerebral ischemia reveals a reperfusion-induced microRNA cluster

• Verfasst von: Uhlmann, Stefan [VerfasserIn]
• Verfasst von: Mracskó, Eva [VerfasserIn]
• Verfasst von: Javidi, Ehsan [VerfasserIn]
• Verfasst von: Hotz-Wagenblatt, Agnes [VerfasserIn]
• Verfasst von: Veltkamp, Roland [VerfasserIn]
• Titel: Genome-wide analysis of the circulating miRNome after cerebral ischemia reveals a reperfusion-induced microRNA cluster
• Verf.angabe: Stefan Uhlmann, Eva Mracsko, Ehsan Javidi, Sarah Lamble, Ana Teixeira, Agnes Hotz-Wagenblatt, Karl-Heinz Glatting, Roland Veltkamp
• E-Jahr: 2017
• Jahr: 13 Feb 2017
• Umfang: 8 S.
• Teil: volume:48
• Teil: year:2017
• Teil: number:3
• Teil: pages:762-769
• Teil: extent:8
• Fussnoten: Gesehen am 28.09.2018
• Titel Quelle: Enthalten in: Stroke
• Ort Quelle: New York, NY : Association, 1970
• Jahr Quelle: 2017
• Band/Heft Quelle: 48(2017), 3, Seite 762-769
• ISSN Quelle: 1524-4628
• DOI: doi:10.1161/STROKEAHA.116.013942
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1581423144

Abstract

Background and Purpose—Circulating microRNAs (miRNAs) are emerging biomarkers for stroke because of their high stability in the bloodstream and association with pathophysiologic conditions. However, the circulating whole-genome miRNAs (miRNome) has not been characterized comprehensively in the acute phase of stroke. Methods—We profiled the circulating miRNome in mouse models of acute ischemic and hemorrhagic stroke by next-generation sequencing. Stroke models were compared with sham-operated and naive mice to identify deregulated circulating miRNAs. Top-ranked miRNAs were validated and further characterized by quantitative reverse transcription polymerase chain reaction. Results—We discovered 24 circulating miRNAs with an altered abundance in the circulation 3 hours after ischemia, whereas the circulating miRNome was not altered after intracerebral hemorrhage compared with sham-operated mice. Among the upregulated miRNAs in ischemia, the top-listed miR-1264/1298/448 cluster was strongly dependent on reper...