Predicting the targeting of tail-anchored proteins to subcellular compartments in mammalian cells

• Verfasst von: Costello, Joseph [VerfasserIn]
• Verfasst von: Islinger, Markus [VerfasserIn]
• Titel: Predicting the targeting of tail-anchored proteins to subcellular compartments in mammalian cells
• Verf.angabe: Joseph L. Costello, Inês G. Castro, Fátima Camões, Tina A. Schrader, Doug McNeall, Jing Yang, Evdokia-Anastasia Giannopoulou, Sílvia Gomes, Vivian Pogenberg, Nina A. Bonekamp, Daniela Ribeiro, Matthias Wilmanns, Gregory Jedd, Markus Islinger and Michael Schrader
• Jahr: 2017
• Umfang: 13 S.
• Fussnoten: Gesehen am 01.10.2018
• Titel Quelle: Enthalten in: Journal of cell science
• Ort Quelle: Cambridge : Company of Biologists Limited, 1853
• Jahr Quelle: 2017
• Band/Heft Quelle: 130(2017), 9, Seite 1675-1687
• ISSN Quelle: 1477-9137
• DOI: doi:10.1242/jcs.200204
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1581459971

Abstract

Skip to Next Section Tail-anchored (TA) proteins contain a single transmembrane domain (TMD) at the C-terminus that anchors them to the membranes of organelles where they mediate critical cellular processes. Accordingly, mutations in genes encoding TA proteins have been identified in a number of severe inherited disorders. Despite the importance of correctly targeting a TA protein to its appropriate membrane, the mechanisms and signals involved are not fully understood. In this study, we identify additional peroxisomal TA proteins, discover more proteins that are present on multiple organelles, and reveal that a combination of TMD hydrophobicity and tail charge determines targeting to distinct organelle locations in mammals. Specifically, an increase in tail charge can override a hydrophobic TMD signal and re-direct a protein from the ER to peroxisomes or mitochondria and vice versa. We show that subtle changes in those parameters can shift TA proteins between organelles, explaining why peroxisomes and mitochondria have many of the same TA proteins. This enabled us to associate characteristic physicochemical parameters in TA proteins with particular organelle groups. Using this classification allowed successful prediction of the location of uncharacterized TA proteins for the first time.