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Status: Bibliographieeintrag

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Verfasst von:Pinder, Nadine [VerfasserIn]   i
 Brenner, Thorsten [VerfasserIn]   i
 Swoboda, Stefanie [VerfasserIn]   i
 Weigand, Markus A. [VerfasserIn]   i
 Hoppe-Tichy, Torsten [VerfasserIn]   i
Titel:Therapeutic drug monitoring of beta-lactam antibiotics
Titelzusatz:influence of sample stability on the analysis of piperacillin, meropenem, ceftazidime and flucloxacillin by HPLC-UV
Verf.angabe:Nadine Pinder, Thorsten Brenner, Stefanie Swoboda, Markus A. Weigand, Torsten Hoppe-Tichy
E-Jahr:2017
Jahr:22 May 2017
Umfang:8 S.
Fussnoten:Gesehen am 02.10.2018
Titel Quelle:Enthalten in: Journal of pharmaceutical and biomedical analysis
Ort Quelle:New York, NY [u.a.] : Science Direct, 1983
Jahr Quelle:2017
Band/Heft Quelle:143(2017), Seite 86-93
ISSN Quelle:1873-264X
Abstract:Introduction Therapeutic drug monitoring (TDM) is a useful tool to optimize antibiotic therapy. Increasing interest in alternative dosing strategies of beta-lactam antibiotics, e.g. continuous or prolonged infusion, require a feasible analytical method for quantification of these antimicrobial agents. However, pre-analytical issues including sample handling and stability are to be considered to provide valuable analytical results. Methods for the simultaneous determination of piperacillin, meropenem, ceftazidime and flucloxacillin, a high performance liquid chromatography (HPLC) method including protein precipitation was established utilizing ertapenem as internal standard. Long-term stability of stock solutions and plasma samples were monitored. Furthermore, whole blood stability of the analytes in heparinized blood tubes was investigated comparing storage under ambient conditions and 2-8°C. Results A calibration range of 5-200μg/ml (piperacillin, ceftazidime, flucloxacillin) and 2-200μg/ml (meropenem) was linear with r2>0.999, precision and inaccuracy were <9% and <11%, respectively. The successfully validated HPLC assay was applied to clinical samples and stability investigations. At −80°C, plasma samples were stable for 9 months (piperacillin, meropenem) or 13 months (ceftazidime, flucloxacillin). Concentrations of the four beta-lactam antibiotics in whole blood tubes were found to remain within specifications for 8h when stored at 2-8°C but not at room temperature. Conclusions The presented method is a rapid and simple option for routine TDM of piperacillin, meropenem, ceftazidime and flucloxacillin. Whereas long-term storage of beta-lactam samples at −80°C is possible for at least 9 months, whole blood tubes are recommended to be kept refrigerated until analysis.
DOI:doi:10.1016/j.jpba.2017.05.037
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: http://dx.doi.org/10.1016/j.jpba.2017.05.037
 Volltext: http://www.sciencedirect.com/science/article/pii/S0731708517302741
 DOI: https://doi.org/10.1016/j.jpba.2017.05.037
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Continuous infusion
 High performance liquid chromatography
 Plasma concentrations
 Pre-analytical stability
 S-Monovette Lithium-Heparin gel
 Whole blood
K10plus-PPN:1581507968
Verknüpfungen:→ Zeitschrift

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