| Online-Ressource |
Verfasst von: | Pinder, Nadine [VerfasserIn]  |
| Brenner, Thorsten [VerfasserIn]  |
| Swoboda, Stefanie [VerfasserIn]  |
| Weigand, Markus A. [VerfasserIn]  |
| Hoppe-Tichy, Torsten [VerfasserIn]  |
Titel: | Therapeutic drug monitoring of beta-lactam antibiotics |
Titelzusatz: | influence of sample stability on the analysis of piperacillin, meropenem, ceftazidime and flucloxacillin by HPLC-UV |
Verf.angabe: | Nadine Pinder, Thorsten Brenner, Stefanie Swoboda, Markus A. Weigand, Torsten Hoppe-Tichy |
E-Jahr: | 2017 |
Jahr: | 22 May 2017 |
Umfang: | 8 S. |
Fussnoten: | Gesehen am 02.10.2018 |
Titel Quelle: | Enthalten in: Journal of pharmaceutical and biomedical analysis |
Ort Quelle: | New York, NY [u.a.] : Science Direct, 1983 |
Jahr Quelle: | 2017 |
Band/Heft Quelle: | 143(2017), Seite 86-93 |
ISSN Quelle: | 1873-264X |
Abstract: | Introduction Therapeutic drug monitoring (TDM) is a useful tool to optimize antibiotic therapy. Increasing interest in alternative dosing strategies of beta-lactam antibiotics, e.g. continuous or prolonged infusion, require a feasible analytical method for quantification of these antimicrobial agents. However, pre-analytical issues including sample handling and stability are to be considered to provide valuable analytical results. Methods for the simultaneous determination of piperacillin, meropenem, ceftazidime and flucloxacillin, a high performance liquid chromatography (HPLC) method including protein precipitation was established utilizing ertapenem as internal standard. Long-term stability of stock solutions and plasma samples were monitored. Furthermore, whole blood stability of the analytes in heparinized blood tubes was investigated comparing storage under ambient conditions and 2-8°C. Results A calibration range of 5-200μg/ml (piperacillin, ceftazidime, flucloxacillin) and 2-200μg/ml (meropenem) was linear with r2>0.999, precision and inaccuracy were <9% and <11%, respectively. The successfully validated HPLC assay was applied to clinical samples and stability investigations. At −80°C, plasma samples were stable for 9 months (piperacillin, meropenem) or 13 months (ceftazidime, flucloxacillin). Concentrations of the four beta-lactam antibiotics in whole blood tubes were found to remain within specifications for 8h when stored at 2-8°C but not at room temperature. Conclusions The presented method is a rapid and simple option for routine TDM of piperacillin, meropenem, ceftazidime and flucloxacillin. Whereas long-term storage of beta-lactam samples at −80°C is possible for at least 9 months, whole blood tubes are recommended to be kept refrigerated until analysis. |
DOI: | doi:10.1016/j.jpba.2017.05.037 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: http://dx.doi.org/10.1016/j.jpba.2017.05.037 |
| Volltext: http://www.sciencedirect.com/science/article/pii/S0731708517302741 |
| DOI: https://doi.org/10.1016/j.jpba.2017.05.037 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | Continuous infusion |
| High performance liquid chromatography |
| Plasma concentrations |
| Pre-analytical stability |
| S-Monovette Lithium-Heparin gel |
| Whole blood |
K10plus-PPN: | 1581507968 |
Verknüpfungen: | → Zeitschrift |
Therapeutic drug monitoring of beta-lactam antibiotics / Pinder, Nadine [VerfasserIn]; 22 May 2017 (Online-Ressource)