Navigation überspringen
Universitätsbibliothek Heidelberg
Status: Bibliographieeintrag

Verfügbarkeit
Standort: ---
Exemplare: ---
heiBIB
 Online-Ressource
Verfasst von:González Avalos, Paula [VerfasserIn]   i
 Deeg, Janosch A. [VerfasserIn]   i
 Spatz, Joachim P. [VerfasserIn]   i
 Dooley, Steven [VerfasserIn]   i
 Eils, Roland [VerfasserIn]   i
 Gladilin, Evgeny [VerfasserIn]   i
 Muernseer, Marlies [VerfasserIn]   i
Titel:Quantification of substrate and cellular strains in stretchable 3D cell cultures
Titelzusatz:an experimental and computational framework
Verf.angabe:P. González‐Avalos, M. Mürnseer, J. Deeg, A. Bachmann, J. Spatz, S. Dooley, R. Eils & E. Gladilin
Jahr:2017
Umfang:11 S.
Fussnoten:Version of Record online: 07 March 2017 ; Gesehen am 04.10.2018
Titel Quelle:Enthalten in: Journal of microscopy
Ort Quelle:Oxford [u.a.] : Wiley-Blackwell, 1969
Jahr Quelle:2017
Band/Heft Quelle:266(2017), 2, Seite 115-125
ISSN Quelle:1365-2818
Abstract:The mechanical cell environment is a key regulator of biological processes . In living tissues, cells are embedded into the 3D extracellular matrix and permanently exposed to mechanical forces. Quantification of the cellular strain state in a 3D matrix is therefore the first step towards understanding how physical cues determine single cell and multicellular behaviour. The majority of cell assays are, however, based on 2D cell cultures that lack many essential features of the in vivo cellular environment. Furthermore, nondestructive measurement of substrate and cellular mechanics requires appropriate computational tools for microscopic image analysis and interpretation. Here, we present an experimental and computational framework for generation and quantification of the cellular strain state in 3D cell cultures using a combination of 3D substrate stretcher, multichannel microscopic imaging and computational image analysis. The 3D substrate stretcher enables deformation of living cells embedded in bead-labelled 3D collagen hydrogels. Local substrate and cell deformations are determined by tracking displacement of fluorescent beads with subsequent finite element interpolation of cell strains over a tetrahedral tessellation. In this feasibility study, we debate diverse aspects of deformable 3D culture construction, quantification and evaluation, and present an example of its application for quantitative analysis of a cellular model system based on primary mouse hepatocytes undergoing transforming growth factor (TGF-β) induced epithelial-to-mesenchymal transition.
DOI:doi:10.1111/jmi.12520
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: http://dx.doi.org/10.1111/jmi.12520
 Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/jmi.12520
 DOI: https://doi.org/10.1111/jmi.12520
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Cell strain quantification
 Collagen hydrogel
 Epithelial-to-mesenchymal transition
 Extracellular matrix
 Live cell imaging
 Stretchable 3D cell culture
K10plus-PPN:1581535058
Verknüpfungen:→ Zeitschrift

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/68311608   QR-Code
zum Seitenanfang