Status: Bibliographieeintrag
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| Verfasst von: | Herzog, Franz [VerfasserIn]  |
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| | Böhringer, Daniel [VerfasserIn] |
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| | Beck, Martin [VerfasserIn] |
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| Titel: | Structural probing of a protein phosphatase 2A network by chemical cross-linking and mass spectrometry |
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| Verf.angabe: | Franz Herzog, Abdullah Kahraman, Daniel Boehringer, Raymond Mak, Andreas Bracher, Thomas Walzthoeni, Alexander Leitner, Martin Beck, Franz-Ulrich Hartl, Nenad Ban, Lars Malmström, Ruedi Aebersold |
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| E-Jahr: | 2012 |
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| Jahr: | 14 September 2012 |
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| Umfang: | 5 S. |
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| Teil: | volume:337 |
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| | year:2012 |
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| | number:6100 |
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| | pages:1348-1352 |
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| | extent:5 |
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| Fussnoten: | Gesehen am 08.10.2018 |
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| Titel Quelle: | Enthalten in: Science |
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| Ort Quelle: | Washington, DC [u.a.] : American Association for the Advancement of Science, 1880 |
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| Jahr Quelle: | 2012 |
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| Band/Heft Quelle: | 337(2012), 6100, Seite 1348-1352 |
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| ISSN Quelle: | 1095-9203 |
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| Abstract: | The identification of proximate amino acids by chemical cross-linking and mass spectrometry (XL-MS) facilitates the structural analysis of homogeneous protein complexes. We gained distance restraints on a modular interaction network of protein complexes affinity-purified from human cells by applying an adapted XL-MS protocol. Systematic analysis of human protein phosphatase 2A (PP2A) complexes identified 176 interprotein and 570 intraprotein cross-links that link specific trimeric PP2A complexes to a multitude of adaptor proteins that control their cellular functions. Spatial restraints guided molecular modeling of the binding interface between immunoglobulin binding protein 1 (IGBP1) and PP2A and revealed the topology of TCP1 ring complex (TRiC) chaperonin interacting with the PP2A regulatory subunit 2ABG. This study establishes XL-MS as an integral part of hybrid structural biology approaches for the analysis of endogenous protein complexes. Spatial restraints revealed by chemical cross-linking and mass spectrometry elucidate the topology of a dynamic signaling network. |
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| DOI: | doi:10.1126/science.1221483 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Kostenfrei: Volltext ; Verlag: http://dx.doi.org/10.1126/science.1221483 |
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| | Kostenfrei: Volltext: http://science.sciencemag.org/content/337/6100/1348 |
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| | DOI: https://doi.org/10.1126/science.1221483 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1581629680 |
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| Verknüpfungen: | → Zeitschrift |
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Structural probing of a protein phosphatase 2A network by chemical cross-linking and mass spectrometry / Herzog, Franz [VerfasserIn]; 14 September 2012 (Online-Ressource)
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