Human bone marrow mesenchymal stem cells for retinal vascular injury

• Verfasst von: Wang, Jin-Da [VerfasserIn]
• Verfasst von: Jonas, Jost B. [VerfasserIn]
• Titel: Human bone marrow mesenchymal stem cells for retinal vascular injury
• Verf.angabe: Jin-Da Wang, Ying An, Jing-Shang Zhang, Xiu-Hua Wan, Jost B. Jonas, Liang Xu and Wei Zhang
• Jahr: 2017
• Jahr des Originals: 2016
• Umfang: 9 S.
• Fussnoten: First published: 03 November 2016 ; Gesehen am 08.10.2018
• Titel Quelle: Enthalten in: Acta ophthalmologica
• Ort Quelle: Oxford : Blackwell, 2008
• Jahr Quelle: 2017
• Band/Heft Quelle: 95(2017), 6, Seite e453-e461
• ISSN Quelle: 1755-3768
• DOI: doi:10.1111/aos.13154
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: blood-retina barrier
• Sach-SW: bone marrow mesenchymal cells
• Sach-SW: diabetic retinopathy
• Sach-SW: neuroprotection
• Sach-SW: neuroregeneration
• Sach-SW: retinal protection
• Sach-SW: retinal regeneration
• Sach-SW: retinal vascular injury
• Sach-SW: retinopathy of prematurity
• K10plus-PPN: 1581647328

Abstract

Purpose: To examine the potential of intravitreally implanted human bone marrow-derived mesenchymal stem cells (BMSCs) to affect vascular repair and the blood-retina barrier in mice and rats with oxygen-induced retinopathy, diabetic retinopathy or retinal ischaemia-reperfusion damage. Methods: Three study groups (oxygen-induced retinopathy group: 18 C57BL/6J mice; diabetic retinopathy group: 15 rats; retinal ischaemia-reperfusion model: 18 rats) received BMSCs injected intravitreally. Control groups (oxygen-induced retinopathy group: 12 C57BL/6J mice; diabetic retinopathy group: 15 rats; retinal ischaemia-reperfusion model: 18 rats) received an intravitreal injection of phosphate-buffered saline. We applied immunohistological techniques to measure retinal vascularization, spectroscopic measurements of intraretinally extravasated fluorescein-conjugated dextran to quantify the blood-retina barrier breakdown, and histomorphometry to assess retinal thickness and retinal ganglion cell count. Results: In the oxygen-induced retinopathy model, the study group with intravitreally injected BMSCs as compared with the control group showed a significantly (p = 0.001) smaller area of retinal neovascularization. In the diabetic retinopathy model, study group and control group did not differ significantly in the amount of intraretinally extravasated dextran. In the retinal ischaemia-reperfusion model, on the 7th day after retina injury, the retina was significantly thicker in the study group than in the control group (p = 0.02), with no significant difference in the retinal ganglion cell count (p = 0.36). Conclusions: Intravitreally implanted human BMSCs were associated with a reduced retinal neovascularization in the oxygen-induced retinopathy model and with a potentially cell preserving effect in the retinal ischaemia-reperfusion model. Intravitreal BMSCs may be of potential interest for the therapy of retinal vascular disorders.