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| Verfasst von: | Wang, Lei [VerfasserIn]  |
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| | Ni, Ming [VerfasserIn] |
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| | Hückelhoven-Krauss, Angela [VerfasserIn] |
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| | Sellner, Leopold [VerfasserIn] |
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| | Hoffmann, Jean-Marc [VerfasserIn] |
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| | Neuber, Brigitte [VerfasserIn] |
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| | Luft, Thomas [VerfasserIn] |
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| | Hegenbart, Ute [VerfasserIn] |
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| | Schönland, Stefan [VerfasserIn] |
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| | Kleist, Christian [VerfasserIn] |
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| | Sill, Martin [VerfasserIn] |
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| | Wuchter, Patrick [VerfasserIn] |
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| | Eckstein, Volker [VerfasserIn] |
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| | Müller-Tidow, Carsten [VerfasserIn] |
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| | Ho, Anthony Dick [VerfasserIn] |
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| | Dreger, Peter [VerfasserIn] |
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| | Schmitt, Michael [VerfasserIn] |
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| | Schmitt, Anita [VerfasserIn] |
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| Titel: | Modulation of B cells and homing marker on NK cells through extracorporeal photopheresis in patients with steroid-refractory/resistant graft-vs.-host disease without hampering anti-viral/anti-leukemic effects |
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| Verf.angabe: | Lei Wang, Ming Ni, Angela Hückelhoven-Krauss, Leopold Sellner, Jean-Marc Hoffmann, Brigitte Neuber, Thomas Luft, Ute Hegenbart, Stefan Schönland, Christian Kleist, Martin Sill, Bao-an Chen, Patrick Wuchter, Volker Eckstein, William Krüger, Inken Hilgendorf, Ronit Yerushalmi, Arnon Nagler, Carsten Müller-Tidow, Anthony D. Ho, Peter Dreger, Michael Schmitt and Anita Schmitt |
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| E-Jahr: | 2018 |
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| Jahr: | 08 October 2018 |
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| Umfang: | 15 S. |
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| Fussnoten: | Gesehen am 11.10.2018 |
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| Titel Quelle: | Enthalten in: Frontiers in immunology |
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| Ort Quelle: | Lausanne : Frontiers Media, 2010 |
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| Jahr Quelle: | 2018 |
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| Band/Heft Quelle: | 9(2018), Artikel-ID 2207 |
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| ISSN Quelle: | 1664-3224 |
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| Abstract: | Graft-versus-host disease (GvHD), a severe complication of allogeneic hematopoietic stem cell transplantation, significantly affects the post-transplant morbidity and mortality. Systemic steroids remain the gold standard for the initial management of GvHD. However, up to 60% of patients will not sufficiently respond to steroids. Extracorporeal photopheresis (ECP), a cell-based immunotherapy, has shown good clinical results in such steroid-refractory/resistant GvHD patients. Given its immunomodulatory, but not global immunosuppressive and steroid-sparing capacity, ECP constitutes an attractive option. In the case of GvHD, the balance of immune cells is destroyed: effector cells are not any longer efficiently controlled by regulatory cells. ECP therapy may restore this balance. However, the precise mechanism and the impact of ECP on anti-viral/anti-leukemic function remain unclear. In this study, 839 ECP treatments were performed on patients with acute GvHD (aGvHD) and chronic GvHD (cGvHD). A comprehensive analysis of effector and regulatory cells in patients under ECP therapy included multi-parametric flow cytometry and tetramer staining, LuminexTM-based cytokine, interferon-γ enzyme-linked immunospot and chromium-51 release assays. Gene profiling of myeloid-derived suppressor cells (MDSCs) was performed by microarray analysis. Immunologically, modulations of effector and regulatory cells as well as proinflammatory cytokines were observed under ECP treatment: (1) GvHD-relevant cell subsets like CD62L+ NK cells and newly defined CD19hiCD20hi B cells were modulated, but (2) quantity and quality of anti-viral/anti-leukemic effector cells were preserved. (3) The development of MDSCs was promoted and switched from an inactivated subset (CD33-CD11b+) to an activated subset (CD33+CD11b+). (4) The frequency of Foxp3+CD4+ regulatory T cells (Tregs) and CD24+CD38hi regulatory B cells was considerably increased in aGvHD patients, and Foxp3+CD8+ Tregs in cGvHD patients. (5) Proinflammatory cytokines like IL-1β, IL-6, IL-8 and TNF-α were significantly reduced. In summary, ECP constitutes an effective immunomodulatory therapy for patients with steroid-refractory/resistant GvHD without impairment of anti-viral/leukemia effects. |
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| DOI: | doi:10.3389/fimmu.2018.02207 |
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| URL: | kostenfrei: Volltext: http://dx.doi.org/10.3389/fimmu.2018.02207 |
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| | kostenfrei: Volltext: https://www.frontiersin.org/articles/10.3389/fimmu.2018.02207/full |
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| | DOI: https://doi.org/10.3389/fimmu.2018.02207 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | (1) GvHD |
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| | (2) ECP |
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| | (3) immunomodulation |
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| | (4) regulatory cells |
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| | (5) pro-inflammatory cytokines |
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| | (6) effector cells |
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| | (7) anti-viral effect |
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| | (8) anti-leukemic effect |
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| K10plus-PPN: | 1581787553 |
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| Verknüpfungen: | → Zeitschrift |
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| Lokale URL UB: |  Zum Volltext |
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Modulation of B cells and homing marker on NK cells through extracorporeal photopheresis in patients with steroid-refractory/resistant graft-vs.-host disease without hampering anti-viral/anti-leukemic effects / Wang, Lei [VerfasserIn]; 08 October 2018 (Online-Ressource)
