UNC-45A is required for neurite extension via controlling NMII activation

• Verfasst von: Iizuka, Yoshie [VerfasserIn]
• Verfasst von: Hellweg, Raffaele [VerfasserIn]
• Verfasst von: Schütz, Florian [VerfasserIn]
• Titel: UNC-45A is required for neurite extension via controlling NMII activation
• Verf.angabe: Yoshie Iizuka, Ashley Mooneyham, Andrew Sieben, Kevin Chen, Makayla Maile, Raffaele Hellweg, Florian Schütz, Kebebush Teckle, Timothy Starr, Venugopal Thayanithy, Rachel Isaksson Vogel, Emil Lou, Michael K. Lee, Martina Bazzaro, and Paul Forscher
• E-Jahr: 2017
• Jahr: 29 Mar 2017
• Umfang: 10 S.
• Fussnoten: Gesehen am 11.10.2018
• Titel Quelle: Enthalten in: Molecular biology of the cell
• Ort Quelle: Bethesda, Md. : American Society for Cell Biology, 1992
• Jahr Quelle: 2017
• Band/Heft Quelle: 28(2017), 10, Seite 1337-1346
• ISSN Quelle: 1939-4586
• DOI: doi:10.1091/mbc.e16-06-0381
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1581769148

Abstract

UNC-45A is a highly conserved member of the UNC-45/CRO1/She4p family of proteins, which act as chaperones for conventional and nonconventional myosins. NMII mediates contractility and actin-based motility, which are fundamental for proper growth cone motility and neurite extension. The presence and role of UNC-45A in neuronal differentiation have been largely unknown. Here we demonstrate that UNC-45A is a novel growth cone-­localized, NMII-associated component of the multiprotein complex regulating growth cone dynamics. We show that UNC-45A is dispensable for neuron survival but required for neurite elongation. Mechanistically, loss of UNC-45A results in increased levels of NMII activation. Collectively our results provide novel insights into the molecular mechanisms of neurite growth and define UNC-45A as a novel and master regulator of NMII-mediated cellular processes in neurons.