| Online-Ressource |
Verfasst von: | Urlaub, Doris [VerfasserIn]  |
| Höfer, Kristine [VerfasserIn]  |
| Müller, Martha-Lena [VerfasserIn]  |
Titel: | LFA-1 activation in NK cells and their subsets |
Titelzusatz: | influence of receptors, maturation, and cytokine stimulation |
Verf.angabe: | Doris Urlaub, Kristine Höfer, Martha-Lena Müller, and Carsten Watzl |
E-Jahr: | 2017 |
Jahr: | 18 January 2017 |
Umfang: | 8 S. |
Fussnoten: | Gesehen am 15.10.2018 |
Titel Quelle: | Enthalten in: The journal of immunology |
Ort Quelle: | Rockville, Md. : American Association of Immunologists, 1916 |
Jahr Quelle: | 2017 |
Band/Heft Quelle: | 198(2017), 5, Seite 1944-1951 |
ISSN Quelle: | 1550-6606 |
Abstract: | The integrin LFA-1 is essential for efficient activation and for cytotoxicity of NK cells because it initiates the assembly of the immunological synapse and mediates firm adhesion to the target. LFA-1 is also needed to polarize the cytotoxic machinery of the NK cell toward the target cell. The binding affinity and avidity of integrins can be regulated via inside-out signals from other receptors. In this article, we investigate the signals necessary to activate LFA-1 in human NK cells. Our data show that LFA-1 has a low ligand-binding activity in resting human NK cells, but it can be stimulated by triggering activating receptors, such as 2B4 or CD16, or by coactivation of different receptor combinations. Short-term stimulation of freshly isolated NK cells with cytokines, such as IL-15, IL-12, or IL-18, does not activate LFA-1 but increases the responsiveness of the cells to subsequent receptor stimulation. Different NK cell subsets vary in their ability to induce LFA-1 binding activity after activating receptor stimulation. Interestingly, the NK cell subsets that are more mature and possess higher cytotoxic potential also show the highest activation of LFA-1, which correlated with the expression of the small calcium-binding protein S100A4. Our data suggest that regulation of LFA-1 is one reason for the different activity of NK cells during differentiation. |
DOI: | doi:10.4049/jimmunol.1601004 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: http://dx.doi.org/10.4049/jimmunol.1601004 |
| kostenfrei: Volltext: http://www.jimmunol.org/content/198/5/1944 |
| DOI: https://doi.org/10.4049/jimmunol.1601004 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1581894805 |
Verknüpfungen: | → Zeitschrift |
LFA-1 activation in NK cells and their subsets / Urlaub, Doris [VerfasserIn]; 18 January 2017 (Online-Ressource)