Status: Bibliographieeintrag
Standort: ---
Exemplare:
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| Online-Ressource |
Verfasst von: | Kityk, Roman [VerfasserIn]  |
| Kopp, Jürgen [VerfasserIn]  |
| Mayer, Matthias P. [VerfasserIn]  |
Titel: | Molecular mechanism of J-domain-triggered ATP hydrolysis by Hsp70 chaperones |
Verf.angabe: | Roman Kityk, Jürgen Kopp, Matthias P. Mayer |
E-Jahr: | 2018 |
Jahr: | 18 January 2018 |
Jahr des Originals: | 2017 |
Umfang: | 15 S. |
Fussnoten: | Gesehen am 15.10.2018 ; Available online 28 December 2017 |
Titel Quelle: | Enthalten in: Molecular cell |
Ort Quelle: | [Cambridge, Mass.] : Cell Press, 1997 |
Jahr Quelle: | 2018 |
Band/Heft Quelle: | 69(2018), 2, Seite 227-237,e1-e4 |
ISSN Quelle: | 1097-4164 |
Abstract: | Efficient targeting of Hsp70 chaperones to substrate proteins depends on J-domain cochaperones, which in synergism with substrates trigger ATP hydrolysis in Hsp70s and concomitant substrate trapping. We present the crystal structure of the J-domain of Escherichia coli DnaJ in complex with the E. coli Hsp70 DnaK. The J-domain interacts not only with DnaK's nucleotide-binding domain (NBD) but also with its substrate-binding domain (SBD) and packs against the highly conserved interdomain linker. Mutational replacement of contacts between J-domain and SBD strongly reduces the ability of substrates to stimulate ATP hydrolysis in the presence of DnaJ and compromises viability at heat shock temperatures. Our data demonstrate that the J-domain and the substrate do not deliver completely independent signals for ATP hydrolysis, but the J-domain, in addition to its direct influence on Hsp70s catalytic center, makes Hsp70 more responsive for the hydrolysis-inducing signal of the substrate, resulting in efficient substrate trapping. |
DOI: | doi:10.1016/j.molcel.2017.12.003 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
DOI: https://doi.org/10.1016/j.molcel.2017.12.003 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | allostery |
| Hsp40 |
| Hsp70 |
| interdomain communication |
| molecular chaperones |
| protein folding |
K10plus-PPN: | 1581919964 |
Verknüpfungen: | → Zeitschrift |
Molecular mechanism of J-domain-triggered ATP hydrolysis by Hsp70 chaperones / Kityk, Roman [VerfasserIn]; 18 January 2018 (Online-Ressource)
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