Storage duration of autologous stem cell preparations has no impact on hematopoietic recovery after transplantation

• Verfasst von: Kriegsmann, Katharina [VerfasserIn]
• Verfasst von: Kriegsmann, Mark [VerfasserIn]
• Verfasst von: Bruckner, Thomas [VerfasserIn]
• Verfasst von: Hillengaß, Jens [VerfasserIn]
• Verfasst von: Goldschmidt, Hartmut [VerfasserIn]
• Verfasst von: Witzens-Harig, Mathias [VerfasserIn]
• Verfasst von: Ho, Anthony Dick [VerfasserIn]
• Verfasst von: Wuchter, Patrick [VerfasserIn]
• Titel: Storage duration of autologous stem cell preparations has no impact on hematopoietic recovery after transplantation
• Verf.angabe: Katharina Lisenko, Petra Pavel, Mark Kriegsmann, Thomas Bruckner, Jens Hillengass, Hartmut Goldschmidt, Mathias Witzens-Harig, Anthony D. Ho, Patrick Wuchter
• Jahr: 2017
• Umfang: 7 S.
• Fussnoten: Available online 21 December 2016 ; Gesehen am 15.10.2018
• Titel Quelle: Enthalten in: Biology of blood and marrow transplantation
• Ort Quelle: [Erscheinungsort nicht ermittelbar] : Elsevier Health Sciences, 1998
• Jahr Quelle: 2017
• Band/Heft Quelle: 23(2017), 4, Seite 684-690
• ISSN Quelle: 1523-6536
• DOI: doi:10.1016/j.bbmt.2016.12.631
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Autologous blood stem cell transplantation
• Sach-SW: Cryopreservation
• Sach-SW: Long-term storage
• Sach-SW: Multiple myeloma
• Sach-SW: Peripheral blood stem cells
• K10plus-PPN: 1581924836

Abstract

Peripheral blood stem cells (PBSCs) are widely used for autologous blood stem cell transplantation (ABSCT). These cells must be stored for months or even years, usually at temperatures ≤−140°C, until their use. Although several in vitro studies on CD34+ viability and clonogenic assays of PBSCs after long-term storage have been reported, only a few publications have investigated the influence of long-term storage on in vivo hematopoietic reconstitution. In this study, we retrospectively analyzed hematopoietic recovery after storage of PBSCs via controlled-rate freezing (CRF) and cryostorage in 10% DMSO at ≤−140°C in 105 patients with multiple myeloma who received high-dose melphalan before ABSCT. Three groups of PBSC transplantation (n = 247) were delineated based on the storage period: short-term (≤12 months, n = 143), medium-term (>12 and ≤60 months, n = 75), and long-term storage (>60 months, n = 29). A neutrophil increase of ≥.5 × 109/L in medium-term or long-term PBSC cryopreservation groups was observed at day 14 after ABSCT; this increase was comparable to patients who received briefly stored PBSCs (day 15). No negative effect of PBSC storage duration was observed on leucocyte or neutrophil reconstitution. Platelet reconstitutions of ≥20  × 109/L and 50 × 109/L were observed after median times of 10 to 11 and 13 to 14 days after ABSCT, respectively. No influence of PBSC storage duration on platelet recovery of ≥20  × 109/L and ≥50 × 109/L was observed in the 3 storage groups (P = .07, P = .32). The number of previous ABSCTs also had no significant impact upon hematopoietic reconstitution. In conclusion, these results indicate that long-term cryopreservation of PBSC products at vapor nitrogen temperature after CRF does not have a negative effect on hematopoietic recovery even after prolonged storage.