Cell perturbation screens for target identification by RNAi

• Verfasst von: Demir, Kubilay [VerfasserIn]
• Verfasst von: Boutros, Michael [VerfasserIn]
• Titel: Cell perturbation screens for target identification by RNAi
• Verf.angabe: Kubilay Demir, Michael Boutros
• E-Jahr: 2012
• Jahr: 18 Juni 2012
• Umfang: 13 S.
• Fussnoten: Gesehen am 16.10.2018
• Titel Quelle: Enthalten in: Bioinformatics and Drug Discovery
• Ausgabe Quelle: 2nd ed. 2012
• Ort Quelle: Totowa, NJ : Humana Press, 2012
• Jahr Quelle: 2012
• Band/Heft Quelle: (2012), Seite 1-13
• ISBN Quelle: 978-1-61779-965-5
• DOI: doi:10.1007/978-1-61779-965-5_1
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Assay development
• Sach-SW: Drug and RNAi screen design
• Sach-SW: Functional genomics
• Sach-SW: Hit prioritization
• Sach-SW: Homogeneous and high-content screen analysis
• Sach-SW: RNAi libraries
• K10plus-PPN: 1581981090

Abstract

Over the last decade, cell-based screening has become a powerful method in target identification and plays an important role both in basic research and drug discovery. The availability of whole genome sequences and improvements in cell-based screening techniques opened new avenues for high-throughput experiments. Large libraries of RNA interference reagents available for many organisms allow the dissection of broad spectrum of cellular processes. Here, we describe the current state of the large-scale phenotype screening with a focus on cell-based screens. We underline the importance and provide details of screen design, scalability, performance, data analysis, and hit prioritization. Similar to classical high-throughput in vitro screens with defined-target approaches in the past, cell-based screens depend on a successful establishment of robust phenotypic assays, the ability to quantitatively measure phenotypic changes and bioinformatics methods for data analysis, integration, and interpretation.