Oxygenation and adenosine deaminase support growth and proliferation of ex vivo cultured Drosophila wing imaginal discs

• Verfasst von: Straßburger, Katrin [VerfasserIn]
• Verfasst von: Boutros, Michael [VerfasserIn]
• Verfasst von: Teleman, Aurelio A. [VerfasserIn]
• Titel: Oxygenation and adenosine deaminase support growth and proliferation of ex vivo cultured Drosophila wing imaginal discs
• Verf.angabe: Katrin Strassburger, Franziska K. Lorbeer, Marilena Lutz, Fabian Graf, Michael Boutros and Aurelio A. Teleman
• Jahr: 2017
• Umfang: 10 S.
• Fussnoten: Gesehen am 17.10.2018
• Titel Quelle: Enthalten in: Development <Cambridge>
• Ort Quelle: Cambridge : The Company of Biologists, 1953
• Jahr Quelle: 2017
• Band/Heft Quelle: 144(2017), 13, Seite 2529-2538
• ISSN Quelle: 1477-9129
• DOI: doi:10.1242/dev.147538
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1582027498

Abstract

The Drosophila wing imaginal disc has been an important model system over past decades for discovering novel biology related to development, signaling and epithelial morphogenesis. Novel experimental approaches have been enabled using a culture setup that allows ex vivo cultures of wing discs. Current setups, however, are not able to sustain both growth and cell-cycle progression of wing discs ex vivo. We discover here a setup that requires both oxygenation of the tissue and adenosine deaminase activity in the medium, and supports both growth and proliferation of wing discs for 9 h. Nonetheless, further work will be required to extend the duration of the culturing and to enable live imaging of the cultured discs in the future.