| Online-Ressource |
Verfasst von: | Seiler, Jana [VerfasserIn]  |
| Breinig, Marco [VerfasserIn]  |
| Caudron-Herger, Maïwen [VerfasserIn]  |
| Polycarpou-Schwarz, Maria [VerfasserIn]  |
| Boutros, Michael [VerfasserIn]  |
| Diederichs, Sven [VerfasserIn]  |
Titel: | The lncRNA VELUCT strongly regulates viability of lung cancer cells despite its extremely low abundance |
Verf.angabe: | Jana Seiler, Marco Breinig, Maïwen Caudron-Herger, Maria Polycarpou-Schwarz, Michael Boutros and Sven Diederichs |
E-Jahr: | 2017 |
Jahr: | 04 February 2017 |
Umfang: | 12 S. |
Fussnoten: | Gesehen am 17.10.2018 |
Titel Quelle: | Enthalten in: Nucleic acids research |
Ort Quelle: | Oxford : Oxford Univ. Press, 1974 |
Jahr Quelle: | 2017 |
Band/Heft Quelle: | 45(2017), 9, Seite 5458-5469 |
ISSN Quelle: | 1362-4962 |
Abstract: | Little is known about the function of most non-coding RNAs (ncRNAs). The majority of long ncRNAs (lncRNAs) is expressed at very low levels and it is a matter of intense debate whether these can be of functional relevance. Here, we identified lncRNAs regulating the viability of lung cancer cells in a highthroughput RNA interference screen. Based on our previous expression profiling, we designed an siRNA library targeting 638 lncRNAs upregulated in human cancer. In a functional siRNA screen analyzing the viability of lung cancer cells, the most prominent hit was a novel lncRNA which we called Viability Enhancing LUng Cancer Transcript (VELUCT). In silico analyses confirmed the non-coding properties of the transcript. Surprisingly, VELUCT was below the detection limit in total RNA from NCI-H460 cells by RT-qPCR as well as RNA-Seq, but was robustly detected in the chromatin-associated RNA fraction. It is an extremely low abundant lncRNA with an RNA copy number of less than one copy per cell. Blocking transcription with actinomycin D revealed that VELUCT RNA was highly unstable which may partially explain its low steady-state concentration. Despite its extremely low abundance, loss-of-function of VELUCT with three independent experimental approaches in three different lung cancer cell lines led to a significant reduction of cell viability: Next to four individual siRNAs, also two complex siPOOLs as well as two antisense oligonucleotides confirmed the strong and specific phenotype. |
DOI: | doi:10.1093/nar/gkx076 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: http://dx.doi.org/10.1093/nar/gkx076 |
| Volltext: https://academic.oup.com/nar/article/45/9/5458/2970150 |
| DOI: https://doi.org/10.1093/nar/gkx076 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1582030634 |
Verknüpfungen: | → Zeitschrift |
¬The¬ lncRNA VELUCT strongly regulates viability of lung cancer cells despite its extremely low abundance / Seiler, Jana [VerfasserIn]; 04 February 2017 (Online-Ressource)