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Verfasst von:Seiler, Jana [VerfasserIn]   i
 Breinig, Marco [VerfasserIn]   i
 Caudron-Herger, Maïwen [VerfasserIn]   i
 Polycarpou-Schwarz, Maria [VerfasserIn]   i
 Boutros, Michael [VerfasserIn]   i
 Diederichs, Sven [VerfasserIn]   i
Titel:The lncRNA VELUCT strongly regulates viability of lung cancer cells despite its extremely low abundance
Verf.angabe:Jana Seiler, Marco Breinig, Maïwen Caudron-Herger, Maria Polycarpou-Schwarz, Michael Boutros and Sven Diederichs
E-Jahr:2017
Jahr:04 February 2017
Umfang:12 S.
Fussnoten:Gesehen am 17.10.2018
Titel Quelle:Enthalten in: Nucleic acids research
Ort Quelle:Oxford : Oxford Univ. Press, 1974
Jahr Quelle:2017
Band/Heft Quelle:45(2017), 9, Seite 5458-5469
ISSN Quelle:1362-4962
Abstract:Little is known about the function of most non-coding RNAs (ncRNAs). The majority of long ncRNAs (lncRNAs) is expressed at very low levels and it is a matter of intense debate whether these can be of functional relevance. Here, we identified lncRNAs regulating the viability of lung cancer cells in a highthroughput RNA interference screen. Based on our previous expression profiling, we designed an siRNA library targeting 638 lncRNAs upregulated in human cancer. In a functional siRNA screen analyzing the viability of lung cancer cells, the most prominent hit was a novel lncRNA which we called Viability Enhancing LUng Cancer Transcript (VELUCT). In silico analyses confirmed the non-coding properties of the transcript. Surprisingly, VELUCT was below the detection limit in total RNA from NCI-H460 cells by RT-qPCR as well as RNA-Seq, but was robustly detected in the chromatin-associated RNA fraction. It is an extremely low abundant lncRNA with an RNA copy number of less than one copy per cell. Blocking transcription with actinomycin D revealed that VELUCT RNA was highly unstable which may partially explain its low steady-state concentration. Despite its extremely low abundance, loss-of-function of VELUCT with three independent experimental approaches in three different lung cancer cell lines led to a significant reduction of cell viability: Next to four individual siRNAs, also two complex siPOOLs as well as two antisense oligonucleotides confirmed the strong and specific phenotype.
DOI:doi:10.1093/nar/gkx076
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: http://dx.doi.org/10.1093/nar/gkx076
 Volltext: https://academic.oup.com/nar/article/45/9/5458/2970150
 DOI: https://doi.org/10.1093/nar/gkx076
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1582030634
Verknüpfungen:→ Zeitschrift

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