Green- to far-red-emitting fluorogenic tetrazine probes

• Verfasst von: Wieczorek, Achim [VerfasserIn]
• Verfasst von: Werther, Philipp [VerfasserIn]
• Verfasst von: Euchner, Jonas [VerfasserIn]
• Verfasst von: Wombacher, Richard [VerfasserIn]
• Titel: Green- to far-red-emitting fluorogenic tetrazine probes
• Titelzusatz: synthetic access and no-wash protein imaging inside living cells
• Verf.angabe: Achim Wieczorek, Philipp Werther, Jonas Euchner and Richard Wombacher
• Jahr: 2017
• Umfang: 5 S.
• Teil: volume:8
• Teil: year:2017
• Teil: number:2
• Teil: pages:1506-1510
• Teil: extent:5
• Fussnoten: Accepted 19th October 2016 ; Gesehen am 18.10.2018
• Titel Quelle: Enthalten in: Chemical science
• Ort Quelle: Cambridge : RSC, 2010
• Jahr Quelle: 2017
• Band/Heft Quelle: 8(2017), 2, Seite 1506-1510
• ISSN Quelle: 2041-6539
• DOI: doi:10.1039/c6sc03879d
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1582085285

Abstract

Fluorogenic probes for bioorthogonal labeling chemistry are highly beneficial to reduce background signal in fluorescence microscopy imaging., Fluorogenic probes for bioorthogonal labeling chemistry are highly beneficial to reduce background signal in fluorescence microscopy imaging. 1,2,4,5-Tetrazines are known substrates for the bioorthogonal inverse electron demand Diels-Alder reaction (DAinv) and tetrazine substituted fluorophores can exhibit fluorogenic properties. Herein, we report the synthesis of a palette of novel fluorogenic tetrazine dyes derived from widely-used fluorophores that cover the entire emission range from green to far-red. We demonstrate the power of the new fluorogenic probes in fixed and live cell labeling experiments and present the first example of intracellular live cell protein imaging using tetrazine-based probes under no-wash conditions.