Transcription start site associated RNAs in bacteria

• Verfasst von: Yus, Eva [VerfasserIn]
• Verfasst von: Gavin, Anne-Claude [VerfasserIn]
• Verfasst von: Bork, Peer [VerfasserIn]
• Titel: Transcription start site associated RNAs in bacteria
• Verf.angabe: Eva Yus, Marc Güell, Ana P Vivancos, Wei-Hua Chen, María Lluch-Senar, Javier Delgado, Anne-Claude Gavin, Peer Bork and Luis Serrano
• E-Jahr: 2012
• Jahr: 1 January 2012
• Umfang: 7 S.
• Fussnoten: Gesehen am 23.10.2018
• Titel Quelle: Enthalten in: Molecular systems biology
• Ort Quelle: [London] : Nature Publishing Group UK, 2005
• Jahr Quelle: 2012
• Band/Heft Quelle: 8(2012), Artikel-ID 585
• ISSN Quelle: 1744-4292
• DOI: doi:10.1038/msb.2012.16
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1582189889

Abstract

Here, we report the genome-wide identification of small RNAs associated with transcription start sites (TSSs), termed tssRNAs, in Mycoplasma pneumoniae. tssRNAs were also found to be present in a different bacterial phyla, Escherichia coli. Similar to the recently identified promoter-associated tiny RNAs (tiRNAs) in eukaryotes, tssRNAs are associated with active promoters. Evidence suggests that these tssRNAs are distinct from previously described abortive transcription RNAs. ssRNAs have an average size of 45 bases and map exactly to the beginning of cognate full-length transcripts and to cryptic TSSs. Expression of bacterial tssRNAs requires factors other than the standard RNA polymerase holoenzyme. We have found that the RNA polymerase is halted at tssRNA positions in vivo, which may indicate that a pausing mechanism exists to prevent transcription in the absence of genes. These results suggest that small RNAs associated with TSSs could be a universal feature of bacterial transcription.