Cross-talk between phosphorylation and lysine acetylation in a genome-reduced bacterium

• Verfasst von: Noort, Vera van [VerfasserIn]
• Verfasst von: Vonkova, Ivana [VerfasserIn]
• Verfasst von: Betts, Matthew J. [VerfasserIn]
• Verfasst von: Russell, Robert B. [VerfasserIn]
• Verfasst von: Bork, Peer [VerfasserIn]
• Verfasst von: Gavin, Anne-Claude [VerfasserIn]
• Titel: Cross-talk between phosphorylation and lysine acetylation in a genome-reduced bacterium
• Verf.angabe: Vera van Noort, Jan Seebacher, Samuel Bader, Shabaz Mohammed, Ivana Vonkova, Matthew J Betts, Sebastian Kühner, Runjun Kumar, Tobias Maier, Martina O'Flaherty, Vladimir Rybin, Arne Schmeisky, Eva Yus, Jörg Stülke, Luis Serrano, Robert B Russell, Albert JR Heck, Peer Bork, Anne-Claude Gavin
• E-Jahr: 2012
• Jahr: 2012 Feb 28
• Umfang: 16 S.
• Fussnoten: Gesehen am 23.10.2018
• Titel Quelle: Enthalten in: Molecular systems biology
• Ort Quelle: [London] : Nature Publishing Group UK, 2005
• Jahr Quelle: 2012
• Band/Heft Quelle: 8(2012), Artikel-ID 571
• ISSN Quelle: 1744-4292
• DOI: doi:10.1038/msb.2012.4
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1582200343

Abstract

Protein post-translational modifications (PTMs) represent important regulatory states that when combined have been hypothesized to act as molecular codes and to generate a functional diversity beyond genome and transcriptome. We systematically investigate the interplay of protein phosphorylation with other post-transcriptional regulatory mechanisms in the genome-reduced bacterium Mycoplasma pneumoniae. Systematic perturbations by deletion of its only two protein kinases and its unique protein phosphatase identified not only the protein-specific effect on the phosphorylation network, but also a modulation of proteome abundance and lysine acetylation patterns, mostly in the absence of transcriptional changes. Reciprocally, deletion of the two putative N-acetyltransferases affects protein phosphorylation, confirming cross-talk between the two PTMs. The measured M. pneumoniae phosphoproteome and lysine acetylome revealed that both PTMs are very common, that (as in Eukaryotes) they often co-occur within the same protein and that they are frequently observed at interaction interfaces and in multifunctional proteins. The results imply previously unreported hidden layers of post-transcriptional regulation intertwining phosphorylation with lysine acetylation and other mechanisms that define the functional state of a cell.