Hydroxylated fluorescent dyes for live-cell labeling

• Verfasst von: Butkevich, Alexey N. [VerfasserIn]
• Verfasst von: Engelhardt, Johann [VerfasserIn]
• Verfasst von: Hell, Stefan [VerfasserIn]
• Titel: Hydroxylated fluorescent dyes for live-cell labeling
• Titelzusatz: synthesis, spectra and super-resolution STED
• Verf.angabe: Alexey N. Butkevich, Vladimir N. Belov, Kirill Kolmakov, Viktor V. Sokolov, Heydar Shojaei, Sven C. Sidenstein, Dirk Kamin, Jessica Matthias, Rifka Vlijm, Johann Engelhardt, Stefan W. Hell
• E-Jahr: 2017
• Jahr: September 7, 2017
• Umfang: 6 S.
• Teil: volume:23
• Teil: year:2017
• Teil: number:50
• Teil: pages:12114-12119
• Teil: extent:6
• Fussnoten: First published: 29 March 2017 ; Gesehen am 24.10.2018
• Titel Quelle: Enthalten in: Chemistry - a European journal
• Ort Quelle: Weinheim : Wiley-VCH, 1995
• Jahr Quelle: 2017
• Band/Heft Quelle: 23(2017), 50, Seite 12114-12119
• ISSN Quelle: 1521-3765
• DOI: doi:10.1002/chem.201701216
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: dyes/pigments
• Sach-SW: fluorescence
• Sach-SW: living cells
• Sach-SW: optical microscopy
• Sach-SW: rhodamines
• K10plus-PPN: 1582265968

Abstract

Hydroxylated rhodamines, carbopyronines, silico- and germanorhodamines with absorption maxima in the range of 530-640 nm were prepared and applied in specific labeling of living cells. The direct and high-yielding entry to germa- and silaxanthones tolerates the presence of protected heteroatoms and may be considered for the syntheses of various sila- and germafluoresceins, as well as -rhodols. Application in stimulated emission depletion (STED) fluorescence microscopy revealed a resolution of 50-75 nm in one- and two-color imaging of vimentin-HaloTag fused protein and native tubulin. The established structure-property relationships allow for prediction of the spectral properties and the positions of spirolactone/zwitterion equilibria for the new analogues of rhodamines, carbo-, silico-, and germanorhodamines using simple additive schemes.