Cell-permeant large stokes shift dyes for transfection-free multicolor nanoscopy

• Verfasst von: Butkevich, Alexey N. [VerfasserIn]
• Verfasst von: Hell, Stefan [VerfasserIn]
• Titel: Cell-permeant large stokes shift dyes for transfection-free multicolor nanoscopy
• Verf.angabe: Alexey N. Butkevich, Gražvydas Lukinavičius, Elisa D’Este, and Stefan W. Hell
• E-Jahr: 2017
• Jahr: 13 September 2017
• Umfang: 4 S.
• Fussnoten: Published online 30 August 2017 ; Gesehen am 25.10.2018
• Titel Quelle: Enthalten in: American Chemical SocietyJournal of the American Chemical Society
• Ort Quelle: Washington, DC : ACS Publications, 1879
• Jahr Quelle: 2017
• Band/Heft Quelle: 139(2017), 36, Seite 12378-12381
• ISSN Quelle: 1520-5126
• DOI: doi:10.1021/jacs.7b06412
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1582281092

Abstract

We designed cell-permeant red-emitting fluorescent dye labels with >140 nm Stokes shifts based on 9-iminoanthrone, 9-imino-10-silaxanthone, and 9-imino-10-germaxanthone fluorophores. The corresponding probes selectively targeting mitochondria, lysosomes, and F-actin demonstrate low toxicity and enable stimulated emission depletion (STED) nanoscopy in neurons, human fibroblasts, U2OS, and HeLa cells. In combination with known small Stokes shift dyes, our probes allow live-cell three-color STED nanoscopy of endogenous targets on popular setups with 775 nm STED wavelength.