| Online-Ressource |
Verfasst von: | Reddy Alla, Suneel [VerfasserIn]  |
| Hell, Stefan [VerfasserIn]  |
Titel: | Stable positioning of Unc13 restricts synaptic vesicle fusion to defined release sites to promote synchronous neurotransmission |
Verf.angabe: | Suneel Reddy-Alla, Mathias A. Böhme, Eric Reynolds, Christina Beis, Andreas T. Grasskamp, Malou M. Mampell, Marta Maglione, Meida Jusyte, Ulises Rey, Husam Babikir, Anthony W. McCarthy, Christine Quentin, Tanja Matkovic, Dominique Dufour Bergeron, Zeeshan Mushtaq, Fabian Göttfert, David Owald, Thorsten Mielke, Stefan W. Hell, Stephan J. Sigrist, and Alexander M. Walter |
E-Jahr: | 2017 |
Jahr: | 31 August 2017 |
Umfang: | 28 S. |
Fussnoten: | Gesehen am 25.10.2018 |
Titel Quelle: | Enthalten in: Neuron |
Ort Quelle: | [Cambridge, Mass.] : Cell Press, 1988 |
Jahr Quelle: | 2017 |
Band/Heft Quelle: | 95(2017), 6, Seite 1350-1364.e12 |
ISSN Quelle: | 1097-4199 |
Abstract: | Summary Neural information processing depends on precisely timed, Ca2+-activated synaptic vesicle exocytosis from release sites within active zones (AZs), but molecular details are unknown. Here, we identify that the (M)Unc13-family member Unc13A generates release sites and show the physiological relevance of their restrictive AZ targeting. Super-resolution and intravital imaging of Drosophila neuromuscular junctions revealed that (unlike the other release factors Unc18 and Syntaxin-1A) Unc13A was stably and precisely positioned at AZs. Local Unc13A levels predicted single AZ activity. Different Unc13A portions selectively affected release site number, position, and functionality. An N-terminal fragment stably localized to AZs, displaced endogenous Unc13A, and reduced the number of release sites, while a C-terminal fragment generated excessive sites at atypical locations, resulting in reduced and delayed evoked transmission that displayed excessive facilitation. Thus, release site generation by the Unc13A C terminus and their specific AZ localization via the N terminus ensure efficient transmission and prevent ectopic, temporally imprecise release. |
DOI: | doi:10.1016/j.neuron.2017.08.016 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: http://dx.doi.org/10.1016/j.neuron.2017.08.016 |
| Volltext: http://www.sciencedirect.com/science/article/pii/S0896627317307316 |
| DOI: https://doi.org/10.1016/j.neuron.2017.08.016 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | active zone |
| high-pressure freeze electron microscopy |
| imaging |
| local synaptic activity imaging |
| release site |
| scaffolding proteins |
| STED microscopy |
| synapse |
| Unc13 |
| variance-mean analysis |
K10plus-PPN: | 1582298076 |
Verknüpfungen: | → Zeitschrift |
Stable positioning of Unc13 restricts synaptic vesicle fusion to defined release sites to promote synchronous neurotransmission / Reddy Alla, Suneel [VerfasserIn]; 31 August 2017 (Online-Ressource)