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Verfasst von:Reddy Alla, Suneel [VerfasserIn]   i
 Hell, Stefan [VerfasserIn]   i
Titel:Stable positioning of Unc13 restricts synaptic vesicle fusion to defined release sites to promote synchronous neurotransmission
Verf.angabe:Suneel Reddy-Alla, Mathias A. Böhme, Eric Reynolds, Christina Beis, Andreas T. Grasskamp, Malou M. Mampell, Marta Maglione, Meida Jusyte, Ulises Rey, Husam Babikir, Anthony W. McCarthy, Christine Quentin, Tanja Matkovic, Dominique Dufour Bergeron, Zeeshan Mushtaq, Fabian Göttfert, David Owald, Thorsten Mielke, Stefan W. Hell, Stephan J. Sigrist, and Alexander M. Walter
E-Jahr:2017
Jahr:31 August 2017
Umfang:28 S.
Fussnoten:Gesehen am 25.10.2018
Titel Quelle:Enthalten in: Neuron
Ort Quelle:[Cambridge, Mass.] : Cell Press, 1988
Jahr Quelle:2017
Band/Heft Quelle:95(2017), 6, Seite 1350-1364.e12
ISSN Quelle:1097-4199
Abstract:Summary Neural information processing depends on precisely timed, Ca2+-activated synaptic vesicle exocytosis from release sites within active zones (AZs), but molecular details are unknown. Here, we identify that the (M)Unc13-family member Unc13A generates release sites and show the physiological relevance of their restrictive AZ targeting. Super-resolution and intravital imaging of Drosophila neuromuscular junctions revealed that (unlike the other release factors Unc18 and Syntaxin-1A) Unc13A was stably and precisely positioned at AZs. Local Unc13A levels predicted single AZ activity. Different Unc13A portions selectively affected release site number, position, and functionality. An N-terminal fragment stably localized to AZs, displaced endogenous Unc13A, and reduced the number of release sites, while a C-terminal fragment generated excessive sites at atypical locations, resulting in reduced and delayed evoked transmission that displayed excessive facilitation. Thus, release site generation by the Unc13A C terminus and their specific AZ localization via the N terminus ensure efficient transmission and prevent ectopic, temporally imprecise release.
DOI:doi:10.1016/j.neuron.2017.08.016
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: http://dx.doi.org/10.1016/j.neuron.2017.08.016
 Volltext: http://www.sciencedirect.com/science/article/pii/S0896627317307316
 DOI: https://doi.org/10.1016/j.neuron.2017.08.016
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:active zone
 high-pressure freeze electron microscopy
 imaging
 local synaptic activity imaging
 release site
 scaffolding proteins
 STED microscopy
 synapse
 Unc13
 variance-mean analysis
K10plus-PPN:1582298076
Verknüpfungen:→ Zeitschrift

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