Isolation and characterization of circulating tumor cells using a novel workflow combining the CellSearch® system and the CellCelector™

• Verfasst von: Neumann, Martin H. D. [VerfasserIn]
• Verfasst von: Endris, Volker [VerfasserIn]
• Verfasst von: Weichert, Wilko [VerfasserIn]
• Titel: Isolation and characterization of circulating tumor cells using a novel workflow combining the CellSearch® system and the CellCelector™
• Verf.angabe: Martin Horst Dieter Neumann, Helen Schneck, Yvonne Decker, Susanne Schömer, André Franken, Volker Endris, Nicole Pfarr, Wilko Weichert, Dieter Niederacher, Tanja Fehm, Hans Neubauer
• Jahr: 2017
• Umfang: 8 S.
• Fussnoten: Published online May 17, 2016 ; Gesehen am 26.10.2018
• Titel Quelle: Enthalten in: Biotechnology progress
• Ort Quelle: Malden, MA : Wiley, 1985
• Jahr Quelle: 2017
• Band/Heft Quelle: 33(2017), 1, Seite 125-132
• ISSN Quelle: 1520-6033
• DOI: doi:10.1002/btpr.2294
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: CellCelector™
• Sach-SW: CellSearch®
• Sach-SW: circulating tumor cells
• Sach-SW: single cell analysis
• K10plus-PPN: 1582330336

Abstract

Circulating tumor cells (CTC) are rare cells which have left the primary tumor to enter the blood stream. Although only a small CTC subgroup is capable of extravasating, the presence of CTCs is associated with an increased risk of metastasis and a shorter overall survival. Understanding the heterogeneous CTC biology will optimize treatment decisions and will thereby improve patient outcome. For this, robust workflows for detection and isolation of CTCs are urgently required. Here, we present a workflow to characterize CTCs by combining the advantages of both the CellSearch® and the CellCelector™ micromanipulation system.