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Verfasst von:Imber, Marcel [VerfasserIn]   i
 Thärichen, Lena [VerfasserIn]   i
 Kolšek, Katra [VerfasserIn]   i
 Gräter, Frauke [VerfasserIn]   i
Titel:Protein S-Bacillithiolation functions in thiol protection and redox regulation of the glyceraldehyde-3-phosphate dehydrogenase Gap in Staphylococcus aureus under hypochlorite stress
Verf.angabe:Marcel Imber, Nguyen Thi Thu Huyen, Agnieszka J. Pietrzyk-Brzezinska, Vu Van Loi, Melanie Hillion, Jörg Bernhardt, Lena Thärichen, Katra Kolšek, Malek Saleh, Chris J. Hamilton, Lorenz Adrian, Frauke Gräter, Markus C. Wahl, and Haike Antelmann
Jahr:2018
Jahr des Originals:2017
Umfang:21 S.
Fussnoten:Published online: 2017 Jan 18 ; Gesehen am 31.10.2018
Titel Quelle:Enthalten in: Antioxidants & redox signaling
Ort Quelle:Larchmont, NY : Liebert, 1999
Jahr Quelle:2018
Band/Heft Quelle:28(2018), 6, Seite 410-430
ISSN Quelle:1557-7716
Abstract:Aims: Bacillithiol (BSH) is the major low-molecular-weight thiol of the human pathogen Staphylococcus aureus. In this study, we used OxICAT and Voronoi redox treemaps to quantify hypochlorite-sensitive protein thiols in S. aureus USA300 and analyzed the role of BSH in protein S-bacillithiolation., Results: The OxICAT analyses enabled the quantification of 228 Cys residues in the redox proteome of S. aureus USA300. Hypochlorite stress resulted in >10% increased oxidation of 58 Cys residues (25.4%) in the thiol redox proteome. Among the highly oxidized sodium hypochlorite (NaOCl)-sensitive proteins are five S-bacillithiolated proteins (Gap, AldA, GuaB, RpmJ, and PpaC). The glyceraldehyde-3-phosphate (G3P) dehydrogenase Gap represents the most abundant S-bacillithiolated protein contributing 4% to the total Cys proteome. The active site Cys151 of Gap was very sensitive to overoxidation and irreversible inactivation by hydrogen peroxide (H2O2) or NaOCl in vitro. Treatment with H2O2 or NaOCl in the presence of BSH resulted in reversible Gap inactivation due to S-bacillithiolation, which could be regenerated by the bacilliredoxin Brx (SAUSA300_1321) in vitro. Molecular docking was used to model the S-bacillithiolated Gap active site, suggesting that formation of the BSH mixed disulfide does not require major structural changes., Conclusion and Innovation: Using OxICAT analyses, we identified 58 novel NaOCl-sensitive proteins in the pathogen S. aureus that could play protective roles against the host immune defense and include the glycolytic Gap as major target for S-bacillithiolation. S-bacillithiolation of Gap did not require structural changes, but efficiently functions in redox regulation and protection of the active site against irreversible overoxidation in S. aureus. Antioxid. Redox Signal. 28, 410-430.
DOI:doi:10.1089/ars.2016.6897
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: http://dx.doi.org/10.1089/ars.2016.6897
 Volltext: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5791933/
 DOI: https://doi.org/10.1089/ars.2016.6897
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1582456917
Verknüpfungen:→ Zeitschrift

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