Orientation and Ordering in Sequence- and Length-Mismatched Surface-Bound DNA Hybrids

• Verfasst von: Howell, Caitlin [VerfasserIn]
• Verfasst von: Jeyachandran, Yekkoni Lakshmanan [VerfasserIn]
• Verfasst von: Kölsch, Patrick [VerfasserIn]
• Verfasst von: Zharnikov, Michael [VerfasserIn]
• Titel: Orientation and Ordering in Sequence- and Length-Mismatched Surface-Bound DNA Hybrids
• Verf.angabe: Caitlin Howell, Yekkoni Lakshmanan Jeyachandran, Patrick Koelsch, and Michael Zharnikov
• E-Jahr: 2012
• Jahr: 24 May 2012
• Umfang: 8 S.
• Fussnoten: Gesehen am 07.11.2018
• Titel Quelle: Enthalten in: The journal of physical chemistry <Washington, DC> / C
• Ort Quelle: Washington, DC : Soc., 2007
• Jahr Quelle: 2012
• Band/Heft Quelle: 116(2012), 20, Seite 11133-11140
• ISSN Quelle: 1932-7455
• DOI: doi:10.1021/jp302381s
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1582647933

Abstract

The effects of target sequence mismatches and target length mismatches on the orientation and ordering of surface-bound, poly(dT)·poly(dA) hybrids on gold were investigated using a combination of high-resolution X-ray photoelectron spectroscopy and near-edge X-ray absorption fine structure spectroscopy. It was found that the overall orientation of the probe and target strands remained upright and relatively unchanged despite the presence of up to four sequence mismatches in the target strands, although the total number of hybrids decreased with higher numbers of mismatches. Upright probes were also observed in hybrids formed with targets of shorter length, with a greater number of hybrids present in these films as compared to those formed with longer targets. These results suggest that in DNA films of relatively high densities, such as those tested here, the presence of sequence or length mismatches in target strands does not result in a large disruption of orientation and ordering in surface-bound hybrids. This finding may be useful for those seeking to maintain consistent DNA strand orientation for precise nanotechnological applications or those using DNA microarrays for biosensing purposes.