| Online-Ressource |
Verfasst von: | Kumar, Rahul [VerfasserIn]  |
| Ciprianidis, Anja [VerfasserIn]  |
| Theiß, Susanne [VerfasserIn]  |
| Steinbeisser, Herbert [VerfasserIn]  |
| Kaufmann, Lilian [VerfasserIn]  |
Titel: | Nemo-like kinase 1 (Nlk1) and paraxial protocadherin (PAPC) cooperatively control Xenopus gastrulation through regulation of Wnt/planar cell polarity (PCP) signaling |
Verf.angabe: | Rahul Kumar, Anja Ciprianidis, Susanne Theiß, Herbert Steinbeißer, Lilian T. Kaufmann |
Jahr: | 2017 |
Umfang: | 12 S. |
Fussnoten: | Available online 28 October 2016 ; Gesehen am 09.11.2018 |
Titel Quelle: | Enthalten in: Differentiation |
Ort Quelle: | New York, NY [u.a.] : Elsevier, 1973 |
Jahr Quelle: | 2017 |
Band/Heft Quelle: | 93(2017), Seite 27-38 |
ISSN Quelle: | 1432-0436 |
Abstract: | The Wnt/planar cell polarity (PCP) pathway directs cell migration during vertebrate gastrulation and is essential for proper embryonic development. Paraxial protocadherin (PAPC, Gene Symbol pcdh8.2) is an important activator of Wnt/PCP signaling during Xenopus gastrulation, but how PAPC activity is controlled is incompletely understood. Here we show that Nemo-like kinase 1 (Nlk1), an atypical mitogen-activated protein (MAP) kinase, physically associates with the C-terminus of PAPC. This interaction mutually stabilizes both proteins by inhibiting polyubiquitination. The Nlk1 mediated stabilization of PAPC is essential for Wnt/PCP signaling, tissue separation and gastrulation movements. We identified two conserved putative phosphorylation sites in the PAPC C-terminus that are critical for Nlk1 mediated PAPC stabilization and Wnt/PCP regulation. Intriguingly, the kinase activity of Nlk1 itself was not essential for its cooperation with PAPC, suggesting an indirect regulation for example by impeding a different kinase that promotes protein degradation. Overall these results outline a novel, kinase independent role of Nlk1, wherein Nlk1 regulates PAPC stabilization and thereby controls gastrulation movements and Wnt/PCP signaling during development. |
DOI: | doi:10.1016/j.diff.2016.10.002 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: http://dx.doi.org/10.1016/j.diff.2016.10.002 |
| Volltext: http://www.sciencedirect.com/science/article/pii/S0301468116300743 |
| DOI: https://doi.org/10.1016/j.diff.2016.10.002 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | Gastrulation movements |
| Nemo-like kinase |
| Paraxial protocadherin |
| Ubiquitination |
| Wnt/PCP |
K10plus-PPN: | 1582750742 |
Verknüpfungen: | → Zeitschrift |
Nemo-like kinase 1 (Nlk1) and paraxial protocadherin (PAPC) cooperatively control Xenopus gastrulation through regulation of Wnt/planar cell polarity (PCP) signaling / Kumar, Rahul [VerfasserIn]; 2017 (Online-Ressource)