Cryopreservation of ovarian tissue

• Verfasst von: Isachenko, Vladimir [VerfasserIn]
• Verfasst von: Montag, Markus [VerfasserIn]
• Titel: Cryopreservation of ovarian tissue
• Titelzusatz: detailed description of methods for transport, freezing and thawing
• Verf.angabe: V. Isachenko, R. Dittrich, G. Keck, E. Isachenko, G. Rahimi, H. van der Ven, M. Montag, I. Hoffmann, A. Müller, W. Distler, M.W. Beckmann, P. Mallmann
• E-Jahr: 2012
• Jahr: 7.8.2012
• Umfang: 6 S.
• Fussnoten: Gesehen am 21.11.2018
• Titel Quelle: Enthalten in: Geburtshilfe und Frauenheilkunde
• Ort Quelle: Stuttgart : Thieme, 1980
• Jahr Quelle: 2012
• Band/Heft Quelle: 72(2012), 10, Seite 927-932
• ISSN Quelle: 1438-8804
• DOI: doi:10.1055/s-0032-1327812
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1583901620

Abstract

Purpose: In many cases cancer therapy leads to an irreversible reduction or even loss of ovarian reserve. Cryopreservation of ovarian tissue with subsequent thawing and re-transplantation of tissue after the cancer is in remission constitutes a promising method to preserve fertility in women. To date, more than 25 cases of live births after re-transplantation of cryopreserved ovarian tissue have been published worldwide. In Germany the first live birth after re-transplantation of cryopreserved tissue was in 2011. Material and Methods: After surgical removal of ovarian tissue in the Gynaecological Clinic of Dresden University, the tissue was sent to the Gynaecological Clinic of Bonn University in a special transport container at 5 °C and was frozen the next day using 1.5 M dimethyl sulfoxide cryosolution. In 2010 this ovarian tissue was thawed using a sucrose solution in the Gynaecological Clinic of Erlangen University Clinical Centre and was laparoscopically re-transplanted into the patient. Results: The patient became pregnant, the pregnancy was uneventful, and she gave birth to a healthy boy. Conclusion: Freezing of ovarian tissue with subsequent re-transplantation as described here is a viable method to preserve fertility in cancer patients.