Systematic approaches to dissect biological processes in stem cells by image-based screening

• Verfasst von: Erdmann, Gerrit [VerfasserIn]
• Verfasst von: Volz, Hans Christian [VerfasserIn]
• Verfasst von: Boutros, Michael [VerfasserIn]
• Titel: Systematic approaches to dissect biological processes in stem cells by image-based screening
• Verf.angabe: Gerrit Erdmann, H. Christian Volz and Michael Boutros
• E-Jahr: 2012
• Jahr: 01 June 2012
• Umfang: 11 S.
• Fussnoten: Gesehen am 21.11.2018
• Titel Quelle: Enthalten in: Biotechnology journal
• Ort Quelle: Weinheim : Wiley-VCH, 2006
• Jahr Quelle: 2012
• Band/Heft Quelle: 7(2012), 6, Seite 768-778
• ISSN Quelle: 1860-7314
• DOI: doi:10.1002/biot.201200117
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: High-content
• Sach-SW: Imaging
• Sach-SW: RNA interference
• Sach-SW: Screening
• Sach-SW: Stem cells
• K10plus-PPN: 1583933085

Abstract

High-throughput RNAi or small molecule screens have proven to be powerful methodologies for the systematic dissection of cellular processes. In model organisms and cell lines, large-scale screens have identified key components of many cellular pathways and helped to identify novel targets in disease-relevant pathways. Image-based high-content screening has become an increasingly important tool in high-throughput screening, enabling changes in phenotype characteristics, such as cell morphology and cell differentiation, to be monitored. In this review, we discuss the use of image-based screening approaches to explore the behavior of adult, embryonic, and induced pluripotent stem cells. First, we review how current pluripotency and differentiation assays can be adapted to high-throughput formats. We then describe general aspects of image-based screening of cells and present an outlook on challenges for screening stem cells.