| |  Online-Ressource |
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| Verfasst von: | Wang, Wenwen [VerfasserIn]  |
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| | Buß, Eike Christian [VerfasserIn] |
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| | Jauch, Anna [VerfasserIn] |
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| | Eckstein, Volker [VerfasserIn] |
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| | Wuchter, Patrick [VerfasserIn] |
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| | Ho, Anthony Dick [VerfasserIn] |
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| | Lutz, Christoph [VerfasserIn] |
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| Titel: | Hematopoietic stem cells can be separated from leukemic cells in a subgroup of adult acute lymphoblastic leukemia patients |
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| Verf.angabe: | Wenwen Wang, Elena Foerner, Eike Buss, Anna Jauch, Volker Eckstein, Patrick Wuchter, Anthony D. Ho, Christoph Lutz |
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| Umfang: | 9 S. |
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| Fussnoten: | Published online 13 Oct 2016 ; Gesehen am 22.11.2018 |
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| Titel Quelle: | Enthalten in: Leukemia and lymphoma |
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| Jahr Quelle: | 2017 |
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| Band/Heft Quelle: | 58(2017), 6, S. 1446-1454 |
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| ISSN Quelle: | 1029-2403 |
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| Abstract: | In B-cell acute lymphoblastic leukemia (B-ALL) separation of normal hematopoietic stem cells (HSC) has so far been limited to a subgroup of patients. As aldehyde dehydrogenase (ALDH)-activity is enriched in various stem cells we investigated its value for HSC isolation in adult B-ALL. Based on ALDH-activity patients could be stratified in ALDH-numerous (≥1.9% ALDH+ cells) and ALDH-rare (<1.9% ALDH+ cells) cases. In ALDH-rare B-ALL clonal-marker negative HSC could be separated by the CD34+CD38−ALDH+ phenotype, whereas this separation was not possible in ALDH-numerous B-ALL. Functional analysis confirmed the HSC-potential of isolated cells, which were uniformly CD19-negative. However, addition of ALDH-activity further improved HSC-purity. In summary, we provide a method to separate functionally normal HSC from leukemic cells in a subgroup of B-ALL patients that can be identified prospectively. This protocol thereby facilitates comparative analyses of matched HSC and leukemic cells in order to improve our understanding of leukemia evolution. |
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| DOI: | doi:10.1080/10428194.2016.1236378 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Verlag: http://dx.doi.org/10.1080/10428194.2016.1236378 |
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| | Verlag: https://doi.org/10.1080/10428194.2016.1236378 |
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| | DOI: https://doi.org/10.1080/10428194.2016.1236378 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1583951911 |
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| Verknüpfungen: | → Zeitschrift |
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Hematopoietic stem cells can be separated from leukemic cells in a subgroup of adult acute lymphoblastic leukemia patients / Wang, Wenwen [VerfasserIn] (Online-Ressource)
