Impact of enzalutamide and its main metabolite N-desmethyl enzalutamide on pharmacokinetically important drug metabolizing enzymes and drug transporters

• Verfasst von: Weiß, Johanna [VerfasserIn]
• Verfasst von: Kocher, Jutta [VerfasserIn]
• Verfasst von: Mueller, Corina [VerfasserIn]
• Verfasst von: Rosenzweig, Stephanie [VerfasserIn]
• Verfasst von: Theile, Dirk [VerfasserIn]
• Titel: Impact of enzalutamide and its main metabolite N-desmethyl enzalutamide on pharmacokinetically important drug metabolizing enzymes and drug transporters
• Verf.angabe: Johanna Weiss, Jutta Kocher, Corina Mueller, Stephanie Rosenzweig, Dirk Theile
• E-Jahr: 2017
• Jahr: December 2017
• Umfang: 9 S.
• Fussnoten: Gesehen am 22.11.2018
• Titel Quelle: Enthalten in: Biopharmaceutics & drug disposition
• Ort Quelle: New York, NY [u.a.] : Wiley, 1980
• Jahr Quelle: 2017
• Band/Heft Quelle: 38(2017), 9, Seite 517-525
• ISSN Quelle: 1099-081X
• DOI: doi:10.1002/bdd.2103
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: drug interactions
• Sach-SW: drug metabolizing enzymes
• Sach-SW: drug transporters
• Sach-SW: enzalutamide
• Sach-SW: N-desmethyl enzalutamide
• K10plus-PPN: 1583952594

Abstract

Enzalutamide is a new drug against castration-resistant prostate cancer. Recent data indicate profound induction of drug metabolizing enzymes (e.g. cytochrome P450 isoenzyme (CYP) 3A4) but comprehensive in vitro data on other CYP enzymes, drug conjugating enzymes or drug transporters is scarce. Moreover, the mechanisms of induction are poorly investigated and the effects of the active metabolite N-desmethyl enzalutamide are unknown. Using LS180 cells as an induction model and quantitative real-time reverse transcription polymerase chain reaction, our study demonstrated a concentration-dependent induction of CYP1A1, CYP1A2, CYP3A5, CYP3A4, UGT1A3, UGT1A9, ABCB1, ABCC2 and ABCG2 mRNA. Induction of CYP3A4 and ABCB1 was confirmed by Western blot analysis and is likely mediated by activation of the nuclear receptor pregnane x receptor, elucidated by a luciferase-based reporter gene assay. Enzalutamide's main active metabolite N-desmethyl enzalutamide exhibited only weak induction properties. mRNA expression of UGT2B7 was suppressed by enzalutamide and its metabolite. Both compounds are apparently not transported by P-glycoprotein (P-gp) or breast cancer resistance protein (BCRP). N-desmethyl enzalutamide more potently inhibited important drug transporters (P-gp, BCRP, OATPs) than enzalutamide. Taken together, the pharmacokinetics of concurrently administered drugs is likely altered during enzalutamide therapy. Levels of metabolically (mainly CYP3A4) eliminated drugs are expected to be decreased, whereas the abundance of compounds with solely transporter-determined pharmacokinetics (P-gp, OATPs) is likely enhanced.