| |  Online-Ressource |
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| Verfasst von: | Kuhse, Jochen [VerfasserIn]  |
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| | Kalbouneh, Hebah [VerfasserIn] |
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| | Schlicksupp, Andrea [VerfasserIn] |
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| | Nawrotzki, Ralph [VerfasserIn] |
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| | Kirsch, Joachim [VerfasserIn] |
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| Titel: | Phosphorylation of gephyrin in hippocampalneurons by cyclin-dependent kinase CDK5 at Ser-270 is dependent on collybistin |
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| Verf.angabe: | Jochen Kuhse, Heba Kalbouneh, Andrea Schlicksupp, Susanne Mükusch, Ralph Nawrotzki, and Joachim Kirsch |
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| E-Jahr: | 2012 |
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| Jahr: | September 2012 |
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| Umfang: | 15 S. |
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| Fussnoten: | Gesehen am 22.11.2018 |
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| Titel Quelle: | Enthalten in: The journal of biological chemistry |
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| Ort Quelle: | Bethesda, Md. : ASBMB Publications, 1905 |
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| Jahr Quelle: | 2012 |
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| Band/Heft Quelle: | 287(2012), 37, Seite 30952-30966 |
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| ISSN Quelle: | 1083-351X |
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| Abstract: | Gephyrin is a scaffold protein essential for the postsynaptic clustering of inhibitory glycine and different subtypes of GABAA receptors. The cellular and molecular mechanisms involved in gephyrin-mediated receptor clustering are still not well understood. Here we provide evidence that the gephyrin-binding protein collybistin is involved in regulating the phosphorylation of gephyrin. We demonstrate that the widely used monoclonal antibody mAb7a is a phospho-specific antibody that allows the cellular and biochemical analysis of gephyrin phosphorylation at Ser-270. In addition, another neighbored epitope determinant was identified at position Thr-276. Analysis of the double mutant gephyrinT276A,S277A revealed significant reduction in gephyrin cluster formation and altered oligomerization behavior of gephyrin. Moreover, pharmacological inhibition of cyclin-dependent kinases in hippocampal neurons reduced postsynaptic gephyrin mAb7a immunoreactivities. In vitro phosphorylation assays and phosphopeptide competition experiments revealed a phosphorylation at Ser-270 depending on enzyme activities of cyclin-dependent kinases CDK1, -2, or -5. These data indicate that collybistin and cyclin-dependent kinases are involved in regulating the phosphorylation of gephyrin at postsynaptic membrane specializations. |
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| DOI: | doi:10.1074/jbc.M112.349597 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext ; Verlag: http://dx.doi.org/10.1074/jbc.M112.349597 |
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| | kostenfrei: Volltext: http://www.jbc.org/content/287/37/30952 |
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| | DOI: https://doi.org/10.1074/jbc.M112.349597 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | CDK (Cyclin-dependent Kinase) |
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| | GABA Receptors |
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| | Glycine Receptors |
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| | Neurons |
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| | Synapses |
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| K10plus-PPN: | 1583955224 |
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| Verknüpfungen: | → Zeitschrift |
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Phosphorylation of gephyrin in hippocampalneurons by cyclin-dependent kinase CDK5 at Ser-270 is dependent on collybistin / Kuhse, Jochen [VerfasserIn]; September 2012 (Online-Ressource)
