Sequential bottom-up assembly of mechanically stabilized synthetic cells by microfluidics

• Verfasst von: Weiss, Marian [VerfasserIn]
• Verfasst von: Frohnmayer, Johannes [VerfasserIn]
• Verfasst von: Benk, Lucia Theresa [VerfasserIn]
• Verfasst von: Haller, Barbara [VerfasserIn]
• Verfasst von: Janiesch, Jan-Willi [VerfasserIn]
• Verfasst von: Platzman, Ilia [VerfasserIn]
• Verfasst von: Spatz, Joachim P. [VerfasserIn]
• Titel: Sequential bottom-up assembly of mechanically stabilized synthetic cells by microfluidics
• Verf.angabe: Marian Weiss, Johannes Patrick Frohnmayer, Lucia Theresa Benk, Barbara Haller, Jan-Willi Janiesch, Thomas Heitkamp, Michael Börsch, Rafael B. Lira, Rumiana Dimova, Reinhard Lipowsky, Eberhard Bodenschatz, Jean-Christophe Baret, Tanja Vidakovic-Koch, Kai Sundmacher, Ilia Platzman, Joachim P. Spatz
• E-Jahr: 2018
• Jahr: 01 January 2018
• Umfang: 8 S.
• Fussnoten: Published: 16 October 2017 ; Gesehen am 26.11.2018
• Titel Quelle: Enthalten in: Nature materials
• Ort Quelle: Basingstoke : Nature Publishing Group, 2002
• Jahr Quelle: 2018
• Band/Heft Quelle: 17(2018), 1, Seite 89-96
• ISSN Quelle: 1476-4660
• DOI: doi:10.1038/nmat5005
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1584423552

Abstract

Compartments for the spatially and temporally controlled assembly of biological processes are essential towards cellular life. Synthetic mimics of cellular compartments based on lipid-based protocells lack the mechanical and chemical stability to allow their manipulation into a complex and fully functional synthetic cell. Here, we present a high-throughput microfluidic method to generate stable, defined sized liposomes termed ‘droplet-stabilized giant unilamellar vesicles (dsGUVs)’. The enhanced stability of dsGUVs enables the sequential loading of these compartments with biomolecules, namely purified transmembrane and cytoskeleton proteins by microfluidic pico-injection technology. This constitutes an experimental demonstration of a successful bottom-up assembly of a compartment with contents that would not self-assemble to full functionality when simply mixed together. Following assembly, the stabilizing oil phase and droplet shells are removed to release functional self-supporting protocells to an aqueous phase, enabling them to interact with physiologically relevant matrices.