| Online-Ressource |
Verfasst von: | Weiss, Marian [VerfasserIn]  |
| Frohnmayer, Johannes [VerfasserIn]  |
| Benk, Lucia Theresa [VerfasserIn]  |
| Haller, Barbara [VerfasserIn]  |
| Janiesch, Jan-Willi [VerfasserIn]  |
| Platzman, Ilia [VerfasserIn]  |
| Spatz, Joachim P. [VerfasserIn]  |
Titel: | Sequential bottom-up assembly of mechanically stabilized synthetic cells by microfluidics |
Verf.angabe: | Marian Weiss, Johannes Patrick Frohnmayer, Lucia Theresa Benk, Barbara Haller, Jan-Willi Janiesch, Thomas Heitkamp, Michael Börsch, Rafael B. Lira, Rumiana Dimova, Reinhard Lipowsky, Eberhard Bodenschatz, Jean-Christophe Baret, Tanja Vidakovic-Koch, Kai Sundmacher, Ilia Platzman, Joachim P. Spatz |
E-Jahr: | 2018 |
Jahr: | 01 January 2018 |
Umfang: | 8 S. |
Fussnoten: | Published: 16 October 2017 ; Gesehen am 26.11.2018 |
Titel Quelle: | Enthalten in: Nature materials |
Ort Quelle: | Basingstoke : Nature Publishing Group, 2002 |
Jahr Quelle: | 2018 |
Band/Heft Quelle: | 17(2018), 1, Seite 89-96 |
ISSN Quelle: | 1476-4660 |
Abstract: | Compartments for the spatially and temporally controlled assembly of biological processes are essential towards cellular life. Synthetic mimics of cellular compartments based on lipid-based protocells lack the mechanical and chemical stability to allow their manipulation into a complex and fully functional synthetic cell. Here, we present a high-throughput microfluidic method to generate stable, defined sized liposomes termed ‘droplet-stabilized giant unilamellar vesicles (dsGUVs)’. The enhanced stability of dsGUVs enables the sequential loading of these compartments with biomolecules, namely purified transmembrane and cytoskeleton proteins by microfluidic pico-injection technology. This constitutes an experimental demonstration of a successful bottom-up assembly of a compartment with contents that would not self-assemble to full functionality when simply mixed together. Following assembly, the stabilizing oil phase and droplet shells are removed to release functional self-supporting protocells to an aqueous phase, enabling them to interact with physiologically relevant matrices. |
DOI: | doi:10.1038/nmat5005 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: http://dx.doi.org/10.1038/nmat5005 |
| Volltext: https://www.nature.com/articles/nmat5005 |
| DOI: https://doi.org/10.1038/nmat5005 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1584423552 |
Verknüpfungen: | → Zeitschrift |
Sequential bottom-up assembly of mechanically stabilized synthetic cells by microfluidics / Weiss, Marian [VerfasserIn]; 01 January 2018 (Online-Ressource)